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Items: 1 to 50 of 100

1.

How to speed up the polymerase chain reaction.

Bustin SA.

Biomol Detect Quantif. 2017 Jun 20;12:10-14. doi: 10.1016/j.bdq.2017.05.002. eCollection 2017 Jun.

2.

MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.

Bustin SA, Wittwer CT.

Clin Chem. 2017 Sep;63(9):1537-1538. doi: 10.1373/clinchem.2016.268953. Epub 2017 Jun 12. Review. No abstract available.

PMID:
28606913
3.

Reproducibility of biomedical research - The importance of editorial vigilance.

Bustin SA, Huggett JF.

Biomol Detect Quantif. 2017 Feb 21;11:1-3. doi: 10.1016/j.bdq.2017.01.002. eCollection 2017 Mar.

4.

Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh.

Ansari WK, Parvej MS, El Zowalaty ME, Jackson S, Bustin SA, Ibrahim AK, El Zowalaty AE, Rahman MT, Zhang H, Khan MF, Ahamed MM, Rahman MF, Rahman M, Nazir KH, Ahmed S, Hossen ML, Kafi MA, Yamage M, Debnath NC, Ahmed G, Ashour HM, Masudur Rahman M, Noreddin A, Rahman MB.

Vet Microbiol. 2016 Sep 25;193:49-59. doi: 10.1016/j.vetmic.2016.07.025. Epub 2016 Aug 2.

5.

Improving the reliability of peer-reviewed publications: We are all in it together.

Bustin SA, Nolan T.

Biomol Detect Quantif. 2015 Dec 28;7:A1-5. doi: 10.1016/j.bdq.2015.11.002. eCollection 2016 Mar.

6.

Proximity assays for sensitive quantification of proteins.

Greenwood C, Ruff D, Kirvell S, Johnson G, Dhillon HS, Bustin SA.

Biomol Detect Quantif. 2015 May 20;4:10-6. doi: 10.1016/j.bdq.2015.04.002. eCollection 2015 Jun. Review.

7.

Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease.

Elliott TR, Rayment NB, Hudspith BN, Hands RE, Taylor K, Parkes GC, Prescott NJ, Petrovska L, Hermon-Taylor J, Brostoff J, Boussioutas A, Mathew CG, Bustin SA, Sanderson JD.

BMC Gastroenterol. 2015 Jul 3;15:75. doi: 10.1186/s12876-015-0305-3.

8.

RDML-Ninja and RDMLdb for standardized exchange of qPCR data.

Ruijter JM, Lefever S, Anckaert J, Hellemans J, Pfaffl MW, Benes V, Bustin SA, Vandesompele J, Untergasser A; RDML consortium.

BMC Bioinformatics. 2015 Jun 20;16:197. doi: 10.1186/s12859-015-0637-6.

9.

Aspergillus-Specific Lateral-Flow Device and Real-Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis.

Johnson GL, Sarker SJ, Nannini F, Ferrini A, Taylor E, Lass-Flörl C, Mutschlechner W, Bustin SA, Agrawal SG.

J Clin Microbiol. 2015 Jul;53(7):2103-8. doi: 10.1128/JCM.00110-15. Epub 2015 Apr 22.

10.

The reproducibility of biomedical research: Sleepers awake!

Bustin SA.

Biomol Detect Quantif. 2015 Jan 21;2:35-42. eCollection 2014 Dec. Review.

11.

Insufficient demonstration of long-term stability of Aspergillus galactomannan.

Johnson GL, Sarker SJ, Bustin SA, Agrawal SG.

J Clin Microbiol. 2014 Nov;52(11):4118. doi: 10.1128/JCM.02342-14. No abstract available.

12.

Influence of trypsinization and alternative procedures for cell preparation before RNA extraction on RNA integrity.

Vrtačnik P, Kos Š, Bustin SA, Marc J, Ostanek B.

Anal Biochem. 2014 Oct 15;463:38-44. doi: 10.1016/j.ab.2014.06.017. Epub 2014 Jun 28.

PMID:
24983903
13.

Biomarkers for invasive aspergillosis: the challenges continue.

Johnson G, Ferrini A, Dolan SK, Nolan T, Agrawal S, Doyle S, Bustin SA.

Biomark Med. 2014;8(3):429-51. doi: 10.2217/bmm.13.129. Review.

PMID:
24712434
14.

Five years MIQE guidelines: the case of the Arabian countries.

Abdel Nour AM, Azhar E, Damanhouri G, Bustin SA.

PLoS One. 2014 Feb 4;9(2):e88266. doi: 10.1371/journal.pone.0088266. eCollection 2014.

15.

Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results?

Dijkstra JR, van Kempen LC, Nagtegaal ID, Bustin SA.

Mol Oncol. 2014 Jun;8(4):813-8. doi: 10.1016/j.molonc.2013.12.016. Epub 2014 Jan 2. Review.

16.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
17.

Avian influenza: virology, diagnosis and surveillance.

El Zowalaty ME, Bustin SA, Husseiny MI, Ashour HM.

Future Microbiol. 2013 Sep;8(9):1209-27. doi: 10.2217/fmb.13.81. Review.

PMID:
24020746
18.

Significant decline in Galactomannan Signal during storage of clinical serum samples.

Johnson GL, Sarker SJ, Hill K, Tsitsikas DA, Morin A, Bustin SA, Agrawal SG.

Int J Mol Sci. 2013 Jun 24;14(7):12970-7. doi: 10.3390/ijms140712970.

19.

Metasin-an intra-operative RT-qPCR assay to detect metastatic breast cancer in sentinel lymph nodes.

Al-Ramadhani S, Sai-Giridhar P, George D, Gopinath P, Arkoumani E, Jader S, Sundaresan M, Salgado R, Larsimont D, Bustin SA, Sundaresan V.

Int J Mol Sci. 2013 Jun 24;14(7):12931-52. doi: 10.3390/ijms140712931.

20.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

21.

Real-time quantitative PCR, pathogen detection and MIQE.

Johnson G, Nolan T, Bustin SA.

Methods Mol Biol. 2013;943:1-16. doi: 10.1007/978-1-60327-353-4_1.

PMID:
23104279
22.

RNA biomarkers in colorectal cancer.

Bustin SA, Murphy J.

Methods. 2013 Jan;59(1):116-25. doi: 10.1016/j.ymeth.2012.10.003. Epub 2012 Oct 16.

PMID:
23079397
23.

A MIQE-compliant real-time PCR assay for Aspergillus detection.

Johnson GL, Bibby DF, Wong S, Agrawal SG, Bustin SA.

PLoS One. 2012;7(7):e40022. doi: 10.1371/journal.pone.0040022. Epub 2012 Jul 10.

24.

Primer sequence disclosure: a clarification of the MIQE guidelines.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT.

Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18. No abstract available.

25.

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments.

Bustin SA, Beaulieu JF, Huggett J, Jaggi R, Kibenge FS, Olsvik PA, Penning LC, Toegel S.

BMC Mol Biol. 2010 Sep 21;11:74. doi: 10.1186/1471-2199-11-74.

26.

Developments in real-time PCR research and molecular diagnostics.

Bustin SA.

Expert Rev Mol Diagn. 2010 Sep;10(6):713-5. doi: 10.1586/erm.10.65.

PMID:
20843195
27.

Why the need for qPCR publication guidelines?--The case for MIQE.

Bustin SA.

Methods. 2010 Apr;50(4):217-26. doi: 10.1016/j.ymeth.2009.12.006. Epub 2009 Dec 16.

PMID:
20025972
28.

Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase, and vitamin D receptor mRNA in normal and malignant breast tissue.

McCarthy K, Laban C, Bustin SA, Ogunkolade W, Khalaf S, Carpenter R, Jenkins PJ.

Anticancer Res. 2009 Jan;29(1):155-7. Erratum in: Anticancer Res. 2009 Oct;29(10):4315.

29.

Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?

Murphy J, Bustin SA.

Expert Rev Mol Diagn. 2009 Mar;9(2):187-97. doi: 10.1586/14737159.9.2.187. Review.

PMID:
19298142
30.

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

Garson JA, Huggett JF, Bustin SA, Pfaffl MW, Benes V, Vandesompele J, Shipley GL.

AIDS Res Hum Retroviruses. 2009 Mar;25(3):377-8; author reply 379-81. doi: 10.1089/aid.2008.0270. No abstract available.

PMID:
19292592
31.

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT.

Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009 Feb 26.

32.

Altered monocyte cyclo-oxygenase response in non-obese diabetic mice.

Beyan H, Buckley LR, Bustin SA, Yousaf N, Pozzilli P, Leslie RD.

Clin Exp Immunol. 2009 Feb;155(2):304-10. doi: 10.1111/j.1365-2249.2008.03825.x. Epub 2008 Nov 24.

33.

Microsatellite and chromosomal stable colorectal cancers demonstrate poor immunogenicity and early disease recurrence.

Banerjea A, Hands RE, Powar MP, Bustin SA, Dorudi S.

Colorectal Dis. 2009 Jul;11(6):601-8. doi: 10.1111/j.1463-1318.2008.01639.x. Epub 2008 Jul 15.

PMID:
18637931
34.

Molecular medicine, gene-expression profiling and molecular diagnostics: putting the cart before the horse.

Bustin SA.

Biomark Med. 2008 Jun;2(3):201-7. doi: 10.2217/17520363.2.3.201. No abstract available.

35.

Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte.

Sindelka R, Jonák J, Hands R, Bustin SA, Kubista M.

Nucleic Acids Res. 2008 Feb;36(2):387-92. Epub 2007 Nov 26.

36.

Molecular staging of colorectal cancer: new paradigm or waste of time?

Murphy B J, Dorudi S, Bustin SA.

Expert Opin Med Diagn. 2007 Sep;1(1):31-45. doi: 10.1517/17530059.1.1.31.

PMID:
23489267
37.

Quantification of mRNA using real-time RT-PCR.

Nolan T, Hands RE, Bustin SA.

Nat Protoc. 2006;1(3):1559-82.

PMID:
17406449
38.

Altered monocyte cyclooxygenase response to lipopolysaccharide in type 1 diabetes.

Beyan H, Goodier MR, Nawroly NS, Hawa MI, Bustin SA, Ogunkolade WB, Londei M, Yousaf N, Leslie RD.

Diabetes. 2006 Dec;55(12):3439-45. Erratum in: Diabetes. 2007 Feb;56(2):549.

39.

Extended silencing of the insulin-like growth factor-I receptor gene in MCF-7 cells by serial transfection with siRNA.

McVittie C, Khalaf S, Ogunkolade W, McCarthy K, Laban C, Macaulay VM, Carpenter R, Bustin SA, Jenkins P.

Anal Biochem. 2006 Oct 15;357(2):305-7. Epub 2006 Aug 8. No abstract available.

PMID:
16942747
40.

Vitamin D metabolism in peripheral blood mononuclear cells is influenced by chewing "betel nut" (Areca catechu) and vitamin D status.

Ogunkolade WB, Boucher BJ, Bustin SA, Burrin JM, Noonan K, Mannan N, Hitman GA.

J Clin Endocrinol Metab. 2006 Jul;91(7):2612-7. Epub 2006 May 2.

PMID:
16670168
41.

Cyclo-oxygenase-2 (COX-2) mRNA expression and hormone receptor status in breast cancer.

McCarthy K, Bustin SA, Ogunkolade B, Khalaf S, Laban CA, McVittie CJ, Carpenter R, Jenkins PJ.

Eur J Surg Oncol. 2006 Sep;32(7):707-9. Epub 2006 May 2.

PMID:
16650963
42.

SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations.

Nolan T, Hands RE, Ogunkolade W, Bustin SA.

Anal Biochem. 2006 Apr 15;351(2):308-10. Epub 2006 Feb 20. No abstract available.

PMID:
16524557
43.

Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer.

Bustin SA, Mueller R.

Mol Aspects Med. 2006 Apr-Jun;27(2-3):192-223. Epub 2006 Jan 30. Review.

PMID:
16445974
44.

Differential expression of IGF-binding protein-3 in normal and malignant colon and its influence on apoptosis.

Jenkins PJ, Khalaf S, Ogunkolade W, McCarthy K, David T, Hands RE, Davies D, Bustin SA.

Endocr Relat Cancer. 2005 Dec;12(4):891-901.

45.

Immunogenic hsp-70 is overexpressed in colorectal cancers with high-degree microsatellite instability.

Banerjea A, Feakins RM, Nickols CD, Phillips SM, Powar MP, Bustin SA, Dorudi S.

Dis Colon Rectum. 2005 Dec;48(12):2322-8.

PMID:
16258706
46.

Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis.

Bustin SA, Mueller R.

Clin Sci (Lond). 2005 Oct;109(4):365-79. Review.

PMID:
16171460
47.

The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization.

Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GA, Zumla A.

Anal Biochem. 2005 Sep 1;344(1):141-3. No abstract available.

PMID:
16054107
48.
49.

Quantitative real-time RT-PCR--a perspective.

Bustin SA, Benes V, Nolan T, Pfaffl MW.

J Mol Endocrinol. 2005 Jun;34(3):597-601. Review.

PMID:
15956331
50.

The immunogenicity of colorectal cancers with high-degree microsatellite instability.

Banerjea A, Bustin SA, Dorudi S.

World J Surg Oncol. 2005 May 12;3:26.

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