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Items: 1 to 20 of 71


Single-molecule imaging of transcriptionally coupled and uncoupled splicing.

Vargas DY, Shah K, Batish M, Levandoski M, Sinha S, Marras SA, Schedl P, Tyagi S.

Cell. 2011 Nov 23;147(5):1054-65. doi: 10.1016/j.cell.2011.10.024.


Deciphering 3'ss selection in the yeast genome reveals an RNA thermosensor that mediates alternative splicing.

Meyer M, Plass M, Pérez-Valle J, Eyras E, Vilardell J.

Mol Cell. 2011 Sep 16;43(6):1033-9. doi: 10.1016/j.molcel.2011.07.030.


Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast.

Bergkessel M, Whitworth GB, Guthrie C.

RNA. 2011 Aug;17(8):1461-78. doi: 10.1261/rna.2754011. Epub 2011 Jun 22.


Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.

Schmidt U, Basyuk E, Robert MC, Yoshida M, Villemin JP, Auboeuf D, Aitken S, Bertrand E.

J Cell Biol. 2011 May 30;193(5):819-29. doi: 10.1083/jcb.201009012.


Real-time observation of transcription initiation and elongation on an endogenous yeast gene.

Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH.

Science. 2011 Apr 22;332(6028):475-8. doi: 10.1126/science.1202142.


Ordered and dynamic assembly of single spliceosomes.

Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ.

Science. 2011 Mar 11;331(6022):1289-95. doi: 10.1126/science.1198830.


The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.

Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D.

J Cell Biol. 2010 Oct 4;191(1):75-86. doi: 10.1083/jcb.201004030.


Cross-talk in transcription, splicing and chromatin: who makes the first call?

Alexander R, Beggs JD.

Biochem Soc Trans. 2010 Oct;38(5):1251-6. doi: 10.1042/BST0381251. Review.


The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing.

Koodathingal P, Novak T, Piccirilli JA, Staley JP.

Mol Cell. 2010 Aug 13;39(3):385-95. doi: 10.1016/j.molcel.2010.07.014.


Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast.

Abelson J, Hadjivassiliou H, Guthrie C.

Methods Enzymol. 2010;472:31-40. doi: 10.1016/S0076-6879(10)72017-6.


Spliceosome discards intermediates via the DEAH box ATPase Prp43p.

Mayas RM, Maita H, Semlow DR, Staley JP.

Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10020-5. doi: 10.1073/pnas.0906022107. Epub 2010 May 12.


Conformational dynamics of single pre-mRNA molecules during in vitro splicing.

Abelson J, Blanco M, Ditzler MA, Fuller F, Aravamudhan P, Wood M, Villa T, Ryan DE, Pleiss JA, Maeder C, Guthrie C, Walter NG.

Nat Struct Mol Biol. 2010 Apr;17(4):504-12. doi: 10.1038/nsmb.1767. Epub 2010 Mar 21.


Expansion of the eukaryotic proteome by alternative splicing.

Nilsen TW, Graveley BR.

Nature. 2010 Jan 28;463(7280):457-63. doi: 10.1038/nature08909. Review.


Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing.

Lardelli RM, Thompson JX, Yates JR 3rd, Stevens SW.

RNA. 2010 Mar;16(3):516-28. doi: 10.1261/rna.2030510. Epub 2010 Jan 20.


The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome.

Fabrizio P, Dannenberg J, Dube P, Kastner B, Stark H, Urlaub H, Lührmann R.

Mol Cell. 2009 Nov 25;36(4):593-608. doi: 10.1016/j.molcel.2009.09.040.


Prp43 bound at different sites on the pre-rRNA performs distinct functions in ribosome synthesis.

Bohnsack MT, Martin R, Granneman S, Ruprecht M, Schleiff E, Tollervey D.

Mol Cell. 2009 Nov 25;36(4):583-92. doi: 10.1016/j.molcel.2009.09.039.


Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.

Warkocki Z, Odenwälder P, Schmitzová J, Platzmann F, Stark H, Urlaub H, Ficner R, Fabrizio P, Lührmann R.

Nat Struct Mol Biol. 2009 Dec;16(12):1237-43. doi: 10.1038/nsmb.1729. Epub 2009 Nov 22.


Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches.

Chen M, Manley JL.

Nat Rev Mol Cell Biol. 2009 Nov;10(11):741-54. doi: 10.1038/nrm2777. Epub 2009 Sep 23. Review.


The role of exon sequences in C complex spliceosome structure.

Ilagan J, Yuh P, Chalkley RJ, Burlingame AL, Jurica MS.

J Mol Biol. 2009 Nov 27;394(2):363-75. doi: 10.1016/j.jmb.2009.09.019. Epub 2009 Sep 15.


Single molecule enzymology: watching the reaction.

Gershenson A.

Curr Opin Chem Biol. 2009 Oct;13(4):436-42. doi: 10.1016/j.cbpa.2009.06.011.


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