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Development of a highly sensitive genus-specific quantitative reverse transcriptase real-time PCR assay for detection and quantitation of plasmodium by amplifying RNA and DNA of the 18S rRNA genes.

Kamau E, Tolbert LS, Kortepeter L, Pratt M, Nyakoe N, Muringo L, Ogutu B, Waitumbi JN, Ockenhouse CF.

J Clin Microbiol. 2011 Aug;49(8):2946-53. doi: 10.1128/JCM.00276-11. Epub 2011 Jun 8.


Comparative field performance and adherence to test results of four malaria rapid diagnostic tests among febrile patients more than five years of age in Blantyre, Malawi.

Chinkhumba J, Skarbinski J, Chilima B, Campbell C, Ewing V, San Joaquin M, Sande J, Ali D, Mathanga D.

Malar J. 2010 Jul 20;9:209. doi: 10.1186/1475-2875-9-209.


Evaluation of three PCR-based diagnostic assays for detecting mixed Plasmodium infection.

Mixson-Hayden T, Lucchi NW, Udhayakumar V.

BMC Res Notes. 2010 Mar 31;3:88. doi: 10.1186/1756-0500-3-88.


Quantitation of RNA decay in dried blood spots during 20 years of storage.

Gauffin F, Nordgren A, Barbany G, Gustafsson B, Karlsson H.

Clin Chem Lab Med. 2009;47(12):1467-9. doi: 10.1515/CCLM.2009.351.


Diagnostic performance of rapid diagnostic tests versus blood smears for malaria in US clinical practice.

Stauffer WM, Cartwright CP, Olson DA, Juni BA, Taylor CM, Bowers SH, Hanson KL, Rosenblatt JE, Boulware DR.

Clin Infect Dis. 2009 Sep 15;49(6):908-13. doi: 10.1086/605436.


The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT.

Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009 Feb 26.


Evaluating assay precision.

Chesher D.

Clin Biochem Rev. 2008 Aug;29 Suppl 1:S23-6.


Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants.

Safeukui I, Millet P, Boucher S, Melinard L, Fregeville F, Receveur MC, Pistone T, Fialon P, Vincendeau P, Fleury H, Malvy D.

Malar J. 2008 Apr 28;7:70. doi: 10.1186/1475-2875-7-70.


Detection of malaria parasites in mosquitoes from the malaria-endemic area of Chakaria, Bangladesh.

Tangin A, Komichi Y, Wagatsuma Y, Rashidul H, Wataya Y, Kim HS.

Biol Pharm Bull. 2008 Apr;31(4):703-8.


Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species.

Ribaut C, Berry A, Chevalley S, Reybier K, Morlais I, Parzy D, Nepveu F, Benoit-Vical F, Valentin A.

Malar J. 2008 Mar 5;7:45. doi: 10.1186/1475-2875-7-45.


Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis.

Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T.

J Clin Microbiol. 2007 Aug;45(8):2521-8. Epub 2007 Jun 13.


Safety and clinical outcome of experimental challenge of human volunteers with Plasmodium falciparum-infected mosquitoes: an update.

Epstein JE, Rao S, Williams F, Freilich D, Luke T, Sedegah M, de la Vega P, Sacci J, Richie TL, Hoffman SL.

J Infect Dis. 2007 Jul 1;196(1):145-54. Epub 2007 May 29.


Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand.

Coleman RE, Sattabongkot J, Promstaporm S, Maneechai N, Tippayachai B, Kengluecha A, Rachapaew N, Zollner G, Miller RS, Vaughan JA, Thimasarn K, Khuntirat B.

Malar J. 2006 Dec 14;5:121.


Evaluation of a real-time PCR assay for malaria diagnosis in patients from Vietnam and in returned travellers.

Vo TK, Bigot P, Gazin P, Sinou V, De Pina JJ, Huynh DC, Fumoux F, Parzy D.

Trans R Soc Trop Med Hyg. 2007 May;101(5):422-8. Epub 2006 Dec 5.


Thick blood film examination for Plasmodium falciparum malaria has reduced sensitivity and underestimates parasite density.

Bejon P, Andrews L, Hunt-Cooke A, Sanderson F, Gilbert SC, Hill AV.

Malar J. 2006 Nov 8;5:104.


Safety, immunogenicity, and efficacy of prime-boost immunization with recombinant poxvirus FP9 and modified vaccinia virus Ankara encoding the full-length Plasmodium falciparum circumsporozoite protein.

Walther M, Thompson FM, Dunachie S, Keating S, Todryk S, Berthoud T, Andrews L, Andersen RF, Moore A, Gilbert SC, Poulton I, Dubovsky F, Tierney E, Correa S, Huntcooke A, Butcher G, Williams J, Sinden RE, Hill AV.

Infect Immun. 2006 May;74(5):2706-16.


Evaluation of a real-time polymerase chain reaction assay for the diagnosis of malaria in patients from Thailand.

Swan H, Sloan L, Muyombwe A, Chavalitshewinkoon-Petmitr P, Krudsood S, Leowattana W, Wilairatana P, Looareesuwan S, Rosenblatt J.

Am J Trop Med Hyg. 2005 Nov;73(5):850-4. Erratum in: Am J Trop Med Hyg. 2006 Jul;75(1):186.


Quantitative real-time polymerase chain reaction for malaria diagnosis and its use in malaria vaccine clinical trials.

Andrews L, Andersen RF, Webster D, Dunachie S, Walther RM, Bejon P, Hunt-Cooke A, Bergson G, Sanderson F, Hill AV, Gilbert SC.

Am J Trop Med Hyg. 2005 Jul;73(1):191-8.


Real-time PCR for detection and identification of Plasmodium spp.

Mangold KA, Manson RU, Koay ES, Stephens L, Regner M, Thomson RB Jr, Peterson LR, Kaul KL.

J Clin Microbiol. 2005 May;43(5):2435-40.

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