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Items: 1 to 20 of 198

1.

In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins.

Buhrman JS, Rayahin JE, Köllmer M, Gemeinhart RA.

BMC Biotechnol. 2012 Sep 18;12:63. doi: 10.1186/1472-6750-12-63.

2.

Purification of GST-Tagged Proteins.

Schäfer F, Seip N, Maertens B, Block H, Kubicek J.

Methods Enzymol. 2015;559:127-39. doi: 10.1016/bs.mie.2014.11.005. Epub 2015 May 16.

PMID:
26096507
3.

Glutathione-S-transferase-green fluorescent protein fusion protein reveals slow dissociation from high site density beads and measures free GSH.

Tessema M, Simons PC, Cimino DF, Sanchez L, Waller A, Posner RG, Wandinger-Ness A, Prossnitz ER, Sklar LA.

Cytometry A. 2006 May;69(5):326-34.

4.

A novel gigaporous GSH affinity medium for high-speed affinity chromatography of GST-tagged proteins.

Huang Y, Zhang R, Li J, Li Q, Su Z, Ma G.

Protein Expr Purif. 2014 Mar;95:84-91. doi: 10.1016/j.pep.2013.11.004. Epub 2013 Nov 19.

PMID:
24269760
5.

Affinity purification of proteins binding to GST fusion proteins.

Swaffield JC, Johnston SA.

Curr Protoc Mol Biol. 2001 May;Chapter 20:Unit 20.2. doi: 10.1002/0471142727.mb2002s33.

PMID:
18265191
6.

Improved isolation of proteins tagged with glutathione S-transferase.

Vinckier NK, Chworos A, Parsons SM.

Protein Expr Purif. 2011 Feb;75(2):161-4. doi: 10.1016/j.pep.2010.09.006. Epub 2010 Sep 16.

PMID:
20849958
7.
8.

Preparation of soluble GST fusion proteins.

Rebay I, Fehon RG.

Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot4996. doi: 10.1101/pdb.prot4996.

PMID:
20150052
9.
10.

A low-cost affinity purification system using β-1,3-glucan recognition protein and curdlan beads.

Horiuchi M, Takahasi K, Kobashigawa Y, Ochiai M, Inagaki F.

Protein Eng Des Sel. 2012 Aug;25(8):405-13. doi: 10.1093/protein/gzs028. Epub 2012 Jun 15.

11.

Expression screen by enzyme-linked immunofiltration assay designed for high-throughput purification of affinity-tagged proteins.

Kery V, Savage JR, Widjaja K, Blake BK, Conklin DR, Ho YS, Long X, von Rechenberg M, Zarembinski TI, Boniface JJ.

Anal Biochem. 2003 Jun 15;317(2):255-8.

PMID:
12758265
12.

Two-step chromatographic purification of glutathione S-transferase-tagged human papillomavirus type 16 E6 protein and its application for serology.

Xu ML, Kim SC, Kim HJ, Ju W, Kim YH, Kim HJ.

Protein Expr Purif. 2017 Apr;132:19-26. doi: 10.1016/j.pep.2017.01.004. Epub 2017 Jan 9.

PMID:
28089881
13.

Purification of proteins fused to glutathione S-transferase.

Harper S, Speicher DW.

Methods Mol Biol. 2011;681:259-80. doi: 10.1007/978-1-60761-913-0_14.

14.

Preparation of insoluble GST fusion proteins.

Rebay I, Fehon RG.

Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot4997. doi: 10.1101/pdb.prot4997.

PMID:
20150053
16.

Expression and purification of large active GST fusion enzymes.

Deceglie S, Lionetti C, Roberti M, Cantatore P, Polosa PL.

Methods Mol Biol. 2014;1129:169-80. doi: 10.1007/978-1-62703-977-2_15.

PMID:
24648076
17.

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli.

Lee S, Han X, Choi KJ, Ding Y, Choi T, Tak E, Lee J, Ha J, Kim SS, Lee J.

Indian J Biochem Biophys. 2008 Dec;45(6):374-8.

PMID:
19239122
18.

A strategy for purifying glutathione S-transferase in the presence of sodium dodecyl sulfate.

Boisselier E, Audet ML, Cantin L, Salesse C.

Biotechniques. 2011 Sep;51(3):193-4. doi: 10.2144/000113739.

19.

Glutathione-S-transferase-fusion based assays for studying protein-protein interactions.

Vikis HG, Guan KL.

Methods Mol Biol. 2004;261:175-86. Review.

PMID:
15064458

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