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Human CD4+ lymphocytes for antigen quantification: characterization using conventional flow cytometry and mass cytometry.

Wang L, Abbasi F, Ornatsky O, Cole KD, Misakian M, Gaigalas AK, He HJ, Marti GE, Tanner S, Stebbings R.

Cytometry A. 2012 Jul;81(7):567-75. doi: 10.1002/cyto.a.22060.


Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry.

Wang M, Misakian M, He HJ, Bajcsy P, Abbasi F, Davis JM, Cole KD, Turko IV, Wang L.

Clin Proteomics. 2014 Dec 11;11(1):43. doi: 10.1186/1559-0275-11-43.


Discrepancy in measuring CD4 expression on T-lymphocytes using fluorescein conjugates in comparison with unimolar CD4-phycoerythrin conjugates.

Wang L, Abbasi F, Gaigalas AK, Hoffman RA, Flagler D, Marti GE.

Cytometry B Clin Cytom. 2007 Nov;72(6):442-9.


Quantification of cells with specific phenotypes II: determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti-CD4 FITC antibody.

Wang L, Stebbings R, Gaigalas AK, Sutherland J, Kammel M, John M, Roemer B, Kuhne M, Schneider RJ, Braun M, Engel A, Dikshit D, Abbasi F, Marti GE, Sassi M, Revel L, Kim SK, Baradez M, Lekishvili T, Marshall D, Whitby L, Jing W, Ost V, Vonsky M, Neukammer J.

Cytometry A. 2015 Mar;87(3):254-61. doi: 10.1002/cyto.a.22634.


Comparison of fluorescein and phycoerythrin conjugates for quantifying CD20 expression on normal and leukemic B-cells.

Wang L, Abbasi F, Gaigalas AK, Vogt RF, Marti GE.

Cytometry B Clin Cytom. 2006 Nov 15;70(6):410-5.


Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins.

Pollice AA, McCoy JP Jr, Shackney SE, Smith CA, Agarwal J, Burholt DR, Janocko LE, Hornicek FJ, Singh SG, Hartsock RJ.

Cytometry. 1992;13(4):432-44.


Changes in fluorescence intensity of selected leukocyte surface markers following fixation.

Stewart JC, Villasmil ML, Frampton MW.

Cytometry A. 2007 Jun;71(6):379-85.


Fixation and cryopreservation of whole blood and isolated mononuclear cells: Influence of different procedures on lymphocyte subset analysis by flow cytometry.

Pinto LA, Trivett MT, Wallace D, Higgins J, Baseler M, Terabe M, Belyakov IM, Berzofsky JA, Hildesheim A.

Cytometry B Clin Cytom. 2005 Jan;63(1):47-55.


Differential expression of T cell antigens in normal peripheral blood lymphocytes: a quantitative analysis by flow cytometry.

Ginaldi L, Farahat N, Matutes E, De Martinis M, Morilla R, Catovsky D.

J Clin Pathol. 1996 Jul;49(7):539-44.


Whole blood method for simultaneous detection of surface and cytoplasmic antigens by flow cytometry.

Landay A, Jennings C, Forman M, Raynor R.

Cytometry. 1993;14(4):433-40. Erratum in: Cytometry 1993;14(6):698.


Standardization of cytokine flow cytometry assays.

Maecker HT, Rinfret A, D'Souza P, Darden J, Roig E, Landry C, Hayes P, Birungi J, Anzala O, Garcia M, Harari A, Frank I, Baydo R, Baker M, Holbrook J, Ottinger J, Lamoreaux L, Epling CL, Sinclair E, Suni MA, Punt K, Calarota S, El-Bahi S, Alter G, Maila H, Kuta E, Cox J, Gray C, Altfeld M, Nougarede N, Boyer J, Tussey L, Tobery T, Bredt B, Roederer M, Koup R, Maino VC, Weinhold K, Pantaleo G, Gilmour J, Horton H, Sekaly RP.

BMC Immunol. 2005 Jun 24;6:13.


Toward quantitative fluorescence measurements with multicolor flow cytometry.

Wang L, Gaigalas AK, Marti G, Abbasi F, Hoffman RA.

Cytometry A. 2008 Apr;73(4):279-88. doi: 10.1002/cyto.a.20507.


Performance of calibration standards for antigen quantitation with flow cytometry in chronic lymphocytic leukemia.

Rossmann ED, Lenkei R, Lundin J, Mellstedt H, Osterborg A.

Cytometry B Clin Cytom. 2007 Nov;72(6):450-7.

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