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Items: 1 to 20 of 101

1.

A novel bacterial expression method with optimized parameters for very high yield production of triple-labeled proteins.

Murray V, Huang Y, Chen J, Wang J, Li Q.

Methods Mol Biol. 2012;831:1-18. doi: 10.1007/978-1-61779-480-3_1.

PMID:
22167665
2.

Practical protocols for production of very high yields of recombinant proteins using Escherichia coli.

Sivashanmugam A, Murray V, Cui C, Zhang Y, Wang J, Li Q.

Protein Sci. 2009 May;18(5):936-48. doi: 10.1002/pro.102.

3.

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

Züger S, Iwai H.

Nat Biotechnol. 2005 Jun;23(6):736-40. Epub 2005 May 22.

PMID:
15908942
4.

Cost-effective method for the preparation of uniformly labeled myristoylated proteins for NMR measurements.

Kroupa T, Prchal J, Doležal M, Ruml T, Hrabal R.

Protein Expr Purif. 2014 Jul;99:6-9. doi: 10.1016/j.pep.2014.03.005. Epub 2014 Mar 21.

PMID:
24662511
5.

Isotopic labeling of heterologous proteins in the yeast Pichia pastoris and Kluyveromyces lactis.

Sugiki T, Ichikawa O, Miyazawa-Onami M, Shimada I, Takahashi H.

Methods Mol Biol. 2012;831:19-36. doi: 10.1007/978-1-61779-480-3_2.

PMID:
22167666
6.

Isotope labeling in mammalian cells.

Dutta A, Saxena K, Schwalbe H, Klein-Seetharaman J.

Methods Mol Biol. 2012;831:55-69. doi: 10.1007/978-1-61779-480-3_4.

7.

Overexpression and purification of isotopically labeled Escherichia coli MutH for NMR studies.

Dutta A, Rao BJ, Chary KV.

Protein Expr Purif. 2003 Jun;29(2):252-8.

PMID:
12767817
8.

Auto-inducing media for uniform isotope labeling of proteins with (15)N, (13)C and (2)H.

Guthertz N, Klopp J, Winterhalter A, Fernández C, Gossert AD.

J Biomol NMR. 2015 Jun;62(2):169-77. doi: 10.1007/s10858-015-9931-x. Epub 2015 Apr 19.

PMID:
25893498
9.

Efficient isotopic tryptophan labeling of membrane proteins by an indole controlled process conduct.

Berger C, Berndt S, Pichert A, Theisgen S, Huster D.

Biotechnol Bioeng. 2013 Jun;110(6):1681-90. doi: 10.1002/bit.24830. Epub 2013 Jan 17.

PMID:
23297054
10.

Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells.

Takahashi H, Shimada I.

J Biomol NMR. 2010 Jan;46(1):3-10. doi: 10.1007/s10858-009-9377-0. Epub 2009 Sep 29.

PMID:
19787297
11.

Sequential assignment of the triple labelled 30.1 kDa cell-adhesion domain of intimin from enteropathogenic E. coli.

Kelly G, Prasannan S, Daniell S, Frankel G, Dougan G, Connerton I, Matthews S.

J Biomol NMR. 1998 Jul;12(1):189-91. No abstract available.

PMID:
9729797
12.

Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems.

Li Z, Nimtz M, Rinas U.

Appl Microbiol Biotechnol. 2011 Nov;92(4):823-33. doi: 10.1007/s00253-011-3603-x. Epub 2011 Oct 9.

PMID:
21983707
13.

Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris.

Pickford AR, O'Leary JM.

Methods Mol Biol. 2004;278:17-33.

PMID:
15317988
14.

Cell-free protein production for NMR studies.

Takeda M, Kainosho M.

Methods Mol Biol. 2012;831:71-84. doi: 10.1007/978-1-61779-480-3_5.

PMID:
22167669
15.

Cost-effective and uniform (13)C- and (15)N-labeling of the 24-kDa N-terminal domain of the Escherichia coli gyrase B by overexpression in the photoautotrophic cyanobacterium Anabaena sp. PCC 7120.

Desplancq D, Kieffer B, Schmidt K, Posten C, Forster A, Oudet P, Strub JM, Van Dorsselaer A, Weiss E.

Protein Expr Purif. 2001 Oct;23(1):207-17.

PMID:
11570864
17.

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination.

Tyler RC, Sreenath HK, Singh S, Aceti DJ, Bingman CA, Markley JL, Fox BG.

Protein Expr Purif. 2005 Apr;40(2):268-78.

PMID:
15766868
18.

Novel strategies to overcome expression problems encountered with toxic proteins: application to the production of Lac repressor proteins for NMR studies.

Romanuka J, van den Bulke H, Kaptein R, Boelens R, Folkers GE.

Protein Expr Purif. 2009 Oct;67(2):104-12. doi: 10.1016/j.pep.2009.05.008. Epub 2009 May 19.

PMID:
19460439
19.
20.

Isotope labeling in insect cells.

Saxena K, Dutta A, Klein-Seetharaman J, Schwalbe H.

Methods Mol Biol. 2012;831:37-54. doi: 10.1007/978-1-61779-480-3_3.

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