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Items: 1 to 20 of 123

1.

High-level secretion of recombinant monomeric murine and human single-chain Fv antibodies from Drosophila S2 cells.

Gilmartin AA, Lamp B, Rümenapf T, Persson MA, Rey FA, Krey T.

Protein Eng Des Sel. 2012 Feb;25(2):59-66. doi: 10.1093/protein/gzr058. Epub 2011 Dec 12.

2.

Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae.

Jørgensen ML, Friis NA, Just J, Madsen P, Petersen SV, Kristensen P.

Microb Cell Fact. 2014 Jan 15;13:9. doi: 10.1186/1475-2859-13-9.

3.

Construction of an scFv library by enzymatic assembly of V(L) and V(H) genes.

Kato M, Hanyu Y.

J Immunol Methods. 2013 Oct 31;396(1-2):15-22. doi: 10.1016/j.jim.2013.07.003. Epub 2013 Jul 31.

PMID:
23916870
4.

Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis.

Pleckaityte M, Mistiniene E, Lasickiene R, Zvirblis G, Zvirbliene A.

BMC Biotechnol. 2011 Nov 3;11:100. doi: 10.1186/1472-6750-11-100.

5.

Secretion from bacterial versus mammalian cells yields a recombinant scFv with variable folding properties.

Vendel MC, Favis M, Snyder WB, Huang F, Capili AD, Dong J, Glaser SM, Miller BR, Demarest SJ.

Arch Biochem Biophys. 2012 Oct 15;526(2):188-93. doi: 10.1016/j.abb.2011.12.018. Epub 2012 Jan 2.

PMID:
22230329
6.

Construction and molecular characterization of mouse single-chain variable fragment antibodies against Burkholderia mallei and Burkholderia pseudomallei.

Kim HS, Tsai S, Zou N, Lo SC, Wear DJ, Izadjoo MJ.

J Immunol Methods. 2011 Feb 28;365(1-2):101-9. doi: 10.1016/j.jim.2010.12.004. Epub 2010 Dec 21.

PMID:
21172353
7.

A fully human scFv phage display library for rapid antibody fragment reformatting.

Li K, Zettlitz KA, Lipianskaya J, Zhou Y, Marks JD, Mallick P, Reiter RE, Wu AM.

Protein Eng Des Sel. 2015 Oct;28(10):307-16. doi: 10.1093/protein/gzv024. Epub 2015 May 19.

8.

Production in yeast of pseudotype virus-like particles harboring functionally active antibody fragments neutralizing the cytolytic activity of vaginolysin.

Pleckaityte M, Zvirbliene A, Sezaite I, Gedvilaite A.

Microb Cell Fact. 2011 Dec 15;10:109. doi: 10.1186/1475-2859-10-109.

9.
10.

Construction of human nonimmune library and selection of scFvs against IL-33.

Yuan Q, Huang L, Wang X, Wu Y, Gao Y, Li C, Nian S.

Appl Biochem Biotechnol. 2012 Jun;167(3):498-509. doi: 10.1007/s12010-012-9676-x. Epub 2012 May 6.

PMID:
22562552
11.

Stability engineering of anti-EGFR scFv antibodies by rational design of a lambda-to-kappa swap of the VL framework using a structure-guided approach.

Lehmann A, Wixted JH, Shapovalov MV, Roder H, Dunbrack RL Jr, Robinson MK.

MAbs. 2015;7(6):1058-71. doi: 10.1080/19420862.2015.1088618. Epub 2015 Sep 4.

12.

Guided selection of a pan carcinoma specific antibody reveals similar binding characteristics yet structural divergence between the original murine antibody and its human equivalent.

Beiboer SH, Reurs A, Roovers RC, Arends JW, Whitelegg NR, Rees AR, Hoogenboom HR.

J Mol Biol. 2000 Feb 25;296(3):833-49.

PMID:
10677285
13.

One-step expression and purification of single-chain variable antibody fragment using an improved hexahistidine tag phagemid vector.

Zhao Q, Chan YW, Lee SS, Cheung WT.

Protein Expr Purif. 2009 Dec;68(2):190-5. doi: 10.1016/j.pep.2009.08.004. Epub 2009 Aug 13.

PMID:
19683057
14.
15.

Conversion of scFv peptide-binding specificity for crystal chaperone development.

Pai JC, Culver JA, Drury JE, Motani RS, Lieberman RL, Maynard JA.

Protein Eng Des Sel. 2011 May;24(5):419-28. doi: 10.1093/protein/gzq120. Epub 2011 Jan 8.

16.

Selection of scFv Antibody Fragments Binding to Human Blood versus Lymphatic Endothelial Surface Antigens by Direct Cell Phage Display.

Keller T, Kalt R, Raab I, Schachner H, Mayrhofer C, Kerjaschki D, Hantusch B.

PLoS One. 2015 May 20;10(5):e0127169. doi: 10.1371/journal.pone.0127169. eCollection 2015.

17.

A combined strategy improves the solubility of aggregation-prone single-chain variable fragment antibodies.

Sun W, Xie J, Lin H, Mi S, Li Z, Hua F, Hu Z.

Protein Expr Purif. 2012 May;83(1):21-9. doi: 10.1016/j.pep.2012.02.006. Epub 2012 Feb 23.

PMID:
22387083
18.

A novel disulfide-stabilized single-chain variable antibody fragment against rabies virus G protein with enhanced in vivo neutralizing potency.

Duan Y, Gu TJ, Jiang CL, Yuan RS, Zhang HF, Hou HJ, Yu XH, Chen Y, Zhang Y, Wu YG, Kong W.

Mol Immunol. 2012 Jun;51(2):188-96. doi: 10.1016/j.molimm.2012.03.015. Epub 2012 Apr 6.

PMID:
22484084
19.

Development of single-chain variable fragment (scFv) antibodies against hapten benzo[a]pyrene: a binding study.

Karsunke XY, Wang H, Weber E, McLean MD, Niessner R, Hall JC, Knopp D.

Anal Bioanal Chem. 2012 Jan;402(1):499-507. doi: 10.1007/s00216-011-5389-1. Epub 2011 Sep 21.

PMID:
21935598
20.

Development of phage-based single chain Fv antibody reagents for detection of Yersinia pestis.

Lillo AM, Ayriss JE, Shou Y, Graves SW, Bradbury AR, Pavlik P.

PLoS One. 2011;6(12):e27756. doi: 10.1371/journal.pone.0027756. Epub 2011 Dec 8. Erratum in: PLoS One. 2012;7(4): doi/10.1371/annotation/fd1d120c-ff3d-4873-85e1-ee70bf010241. Pavlik, Peter [added]..

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