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Items: 1 to 20 of 100

1.

High throughput microRNA profiling: optimized multiplex qRT-PCR at nanoliter scale on the fluidigm dynamic arrayTM IFCs.

Moltzahn F, Hunkapiller N, Mir AA, Imbar T, Blelloch R.

J Vis Exp. 2011 Aug 3;(54). pii: 2552. doi: 10.3791/2552.

2.

220-plex microRNA expression profile of a single cell.

Tang F, Hajkova P, Barton SC, O'Carroll D, Lee C, Lao K, Surani MA.

Nat Protoc. 2006;1(3):1154-9.

PMID:
17406397
3.

Microfluidic-based multiplex qRT-PCR identifies diagnostic and prognostic microRNA signatures in the sera of prostate cancer patients.

Moltzahn F, Olshen AB, Baehner L, Peek A, Fong L, Stöppler H, Simko J, Hilton JF, Carroll P, Blelloch R.

Cancer Res. 2011 Jan 15;71(2):550-60. doi: 10.1158/0008-5472.CAN-10-1229. Epub 2010 Nov 22.

4.

Monitoring microRNA expression during embryonic stem-cell differentiation using quantitative real-time PCR (qRT-PCR).

Pan X, Murashov AK, Stellwag EJ, Zhang B.

Methods Mol Biol. 2010;650:213-24. doi: 10.1007/978-1-60761-769-3_16.

PMID:
20686954
5.

Quantitative miRNA expression analysis using fluidigm microfluidics dynamic arrays.

Jang JS, Simon VA, Feddersen RM, Rakhshan F, Schultz DA, Zschunke MA, Lingle WL, Kolbert CP, Jen J.

BMC Genomics. 2011 Mar 9;12:144. doi: 10.1186/1471-2164-12-144.

6.

A facile and specific assay for quantifying microRNA by an optimized RT-qPCR approach.

Mei Q, Li X, Meng Y, Wu Z, Guo M, Zhao Y, Fu X, Han W.

PLoS One. 2012;7(10):e46890. doi: 10.1371/journal.pone.0046890. Epub 2012 Oct 5.

7.

Improved RT-PCR Assay to Quantitate the Pri-, Pre-, and Mature microRNAs with Higher Efficiency and Accuracy.

Tong L, Xue H, Xiong L, Xiao J, Zhou Y.

Mol Biotechnol. 2015 Oct;57(10):939-46. doi: 10.1007/s12033-015-9885-y.

PMID:
26294305
8.

Real-time quantification of microRNAs by stem-loop RT-PCR.

Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ.

Nucleic Acids Res. 2005 Nov 27;33(20):e179.

9.

High-throughput approaches for microRNA expression analysis.

Dedeoğlu BG.

Methods Mol Biol. 2014;1107:91-103. doi: 10.1007/978-1-62703-748-8_6.

PMID:
24272433
10.

Variability in microRNA recovery from plasma: Comparison of five commercial kits.

Brunet-Vega A, Pericay C, Quílez ME, Ramírez-Lázaro MJ, Calvet X, Lario S.

Anal Biochem. 2015 Nov 1;488:28-35. doi: 10.1016/j.ab.2015.07.018. Epub 2015 Aug 10.

PMID:
26271186
11.

Quantitative PCR based expression analysis on a nanoliter scale using polymer nano-well chips.

Dahl A, Sultan M, Jung A, Schwartz R, Lange M, Steinwand M, Livak KJ, Lehrach H, Nyarsik L.

Biomed Microdevices. 2007 Jun;9(3):307-14.

PMID:
17203381
12.

Quantitative RT-PCR methods for mature microRNA expression analysis.

Fiedler SD, Carletti MZ, Christenson LK.

Methods Mol Biol. 2010;630:49-64. doi: 10.1007/978-1-60761-629-0_4.

PMID:
20300990
13.

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers.

Balcells I, Cirera S, Busk PK.

BMC Biotechnol. 2011 Jun 25;11:70. doi: 10.1186/1472-6750-11-70.

14.

Rapid, quantitative, reverse transcription PCR in a polymer microfluidic chip.

Saunders DC, Holst GL, Phaneuf CR, Pak N, Marchese M, Sondej N, McKinnon M, Forest CR.

Biosens Bioelectron. 2013 Jun 15;44:222-8. doi: 10.1016/j.bios.2013.01.019. Epub 2013 Jan 18.

PMID:
23434757
15.

Measuring microRNA expression in mouse hematopoietic stem cells.

Hu W, Park CY.

Methods Mol Biol. 2014;1185:121-40. doi: 10.1007/978-1-4939-1133-2_8.

PMID:
25062625
16.

MIR-34c regulates mouse embryonic stem cells differentiation into male germ-like cells through RARg.

Zhang S, Yu M, Liu C, Wang L, Hu Y, Bai Y, Hua J.

Cell Biochem Funct. 2012 Dec;30(8):623-32. doi: 10.1002/cbf.2922. Epub 2012 Oct 24.

PMID:
23097316
17.

Normalization of microRNA quantitative RT-PCR data in reduced scale experimental designs.

Latham GJ.

Methods Mol Biol. 2010;667:19-31. doi: 10.1007/978-1-60761-811-9_2.

PMID:
20827524
18.

Nanoliter droplet array for microRNA detection based on enzymatic stem-loop probes ligation and SYBR Green real-time PCR.

Yu Z, Zhu Y, Zhang Y, Li J, Fang Q, Xi J, Yao B.

Talanta. 2011 Sep 30;85(4):1760-5. doi: 10.1016/j.talanta.2011.06.075. Epub 2011 Jul 7.

PMID:
21872015
19.

MicroRNA expression patterns and function in endodermal differentiation of human embryonic stem cells.

Tzur G, Levy A, Meiri E, Barad O, Spector Y, Bentwich Z, Mizrahi L, Katzenellenbogen M, Ben-Shushan E, Reubinoff BE, Galun E.

PLoS One. 2008;3(11):e3726. doi: 10.1371/journal.pone.0003726. Epub 2008 Nov 18.

20.

Quantitation of CD39 gene expression in pancreatic tissue by real-time polymerase chain reaction.

Loos M, Künzli B, Friess H.

Methods Mol Biol. 2010;576:351-62. doi: 10.1007/978-1-59745-545-9_18.

PMID:
19882271

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