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Items: 1 to 20 of 174

1.

HaloTag-based purification of functional human kinases from mammalian cells.

Ohana RF, Hurst R, Vidugiriene J, Slater MR, Wood KV, Urh M.

Protein Expr Purif. 2011 Apr;76(2):154-64. doi: 10.1016/j.pep.2010.11.014.

PMID:
21129486
2.

HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification.

Ohana RF, Encell LP, Zhao K, Simpson D, Slater MR, Urh M, Wood KV.

Protein Expr Purif. 2009 Nov;68(1):110-20. doi: 10.1016/j.pep.2009.05.010.

PMID:
19464373
3.

Purification of Recombinant Proteins from Cultured Mammalian Cells by HaloTag Technology.

Ohana RF, Hurst R.

Curr Protoc Mol Biol. 2015 Apr 1;110:10.31.1-15. doi: 10.1002/0471142727.mb1031s110.

PMID:
25827085
4.

Methods for detection of protein-protein and protein-DNA interactions using HaloTag.

Urh M, Hartzell D, Mendez J, Klaubert DH, Wood K.

Methods Mol Biol. 2008;421:191-209.

PMID:
18826056
5.

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage.

Lu W, Sun Z, Tang Y, Chen J, Tang F, Zhang J, Liu JN.

J Chromatogr A. 2011 May 6;1218(18):2553-60. doi: 10.1016/j.chroma.2011.02.053.

PMID:
21397239
6.

Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins.

Yamaguchi K, Inoue S, Ohara O, Nagase T.

Methods Mol Biol. 2009;577:121-31. doi: 10.1007/978-1-60761-232-2_10.

PMID:
19718513
8.

Expression and purification of full-length mouse CARM1 from transiently transfected HEK293T cells using HaloTag technology.

Chumanov RS, Kuhn PA, Xu W, Burgess RR.

Protein Expr Purif. 2011 Apr;76(2):145-53. doi: 10.1016/j.pep.2010.11.010.

9.

Orthogonal protein purification facilitated by a small bispecific affinity tag.

Nilvebrant J, Alm T, Hober S.

J Vis Exp. 2012 Jan 16;(59). pii: 3370. doi: 10.3791/3370.

10.

A modified metal-ion affinity chromatography procedure for the purification of histidine-tagged recombinant proteins expressed in Drosophila S2 cells.

Lehr RV, Elefante LC, Kikly KK, O'Brien SP, Kirkpatrick RB.

Protein Expr Purif. 2000 Aug;19(3):362-8.

PMID:
10910726
11.

Comparison of affinity tags for protein purification.

Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S.

Protein Expr Purif. 2005 May;41(1):98-105.

PMID:
15802226
12.

Functional expression of milligram quantities of the synthetic human serotonin transporter gene in a tetracycline-inducible HEK293 cell line.

Takayama H, Sugio S.

Protein Expr Purif. 2011 Apr;76(2):211-20. doi: 10.1016/j.pep.2010.11.015.

PMID:
21129485
13.

HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, Zimprich C, Wood MG, Learish R, Ohana RF, Urh M, Simpson D, Mendez J, Zimmerman K, Otto P, Vidugiris G, Zhu J, Darzins A, Klaubert DH, Bulleit RF, Wood KV.

ACS Chem Biol. 2008 Jun 20;3(6):373-82. doi: 10.1021/cb800025k.

PMID:
18533659
15.

Purification of GFP fusion proteins with high purity and yield by monoclonal antibody-coupled affinity column chromatography.

Zhuang R, Zhang Y, Zhang R, Song C, Yang K, Yang A, Jin B.

Protein Expr Purif. 2008 May;59(1):138-43. doi: 10.1016/j.pep.2008.01.020.

PMID:
18342538
16.

Horse cytokine/IgG fusion proteins--mammalian expression of biologically active cytokines and a system to verify antibody specificity to equine cytokines.

Wagner B, Robeson J, McCracken M, Wattrang E, Antczak DF.

Vet Immunol Immunopathol. 2005 May 1;105(1-2):1-14.

PMID:
15797470
17.

Protein expression strategies for identification of novel target proteins.

Schuster M, Wasserbauer E, Einhauer A, Ortner C, Jungbauer A, Hammerschmid F, Werner G.

J Biomol Screen. 2000 Apr;5(2):89-97.

PMID:
10803608
18.
19.

Tab2, a novel recombinant polypeptide tag offering sensitive and specific protein detection and reliable affinity purification.

Crusius K, Finster S, McClary J, Xia W, Larsen B, Schneider D, Lu HT, Biancalana S, Xuan JA, Newton A, Allen D, Bringmann P, Cobb RR.

Gene. 2006 Oct 1;380(2):111-9.

PMID:
16879932
20.

Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme.

Cass B, Pham PL, Kamen A, Durocher Y.

Protein Expr Purif. 2005 Mar;40(1):77-85.

PMID:
15721774
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