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Items: 1 to 20 of 81

1.

Overexpression and purification of integral membrane proteins in yeast.

Hays FA, Roe-Zurz Z, Stroud RM.

Methods Enzymol. 2010;470:695-707. doi: 10.1016/S0076-6879(10)70029-X. Epub 2010 Mar 1.

PMID:
20946832
2.

Integral Membrane Protein Expression in Saccharomyces cerevisiae.

Boswell-Casteel RC, Johnson JM, Stroud RM, Hays FA.

Methods Mol Biol. 2016;1432:163-86. doi: 10.1007/978-1-4939-3637-3_11.

PMID:
27485336
3.

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.

Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S.

Nat Protoc. 2008;3(5):784-98. doi: 10.1038/nprot.2008.44.

4.

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.

Newstead S, Kim H, von Heijne G, Iwata S, Drew D.

Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13936-41. Epub 2007 Aug 20.

5.

New examples of membrane protein expression and purification using the yeast based Pdr1-3 expression strategy.

Gupta RP, Kueppers P, Schmitt L.

J Biotechnol. 2014 Dec 10;191:158-64. doi: 10.1016/j.jbiotec.2014.07.010. Epub 2014 Jul 15.

PMID:
25036752
6.

Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.

Clark KM, Fedoriw N, Robinson K, Connelly SM, Randles J, Malkowski MG, DeTitta GT, Dumont ME.

Protein Expr Purif. 2010 Jun;71(2):207-23. doi: 10.1016/j.pep.2009.12.012. Epub 2010 Jan 4.

7.
8.

Use of a yeast-based membrane protein expression technology to overexpress drug resistance efflux pumps.

Lamping E, Cannon RD.

Methods Mol Biol. 2010;666:219-50. doi: 10.1007/978-1-60761-820-1_15.

PMID:
20717788
9.

Analysis of membrane protein interactions using yeast-based technologies.

Stagljar I, Fields S.

Trends Biochem Sci. 2002 Nov;27(11):559-63. Review.

PMID:
12417131
10.

Expression and purification of the cystic fibrosis transmembrane conductance regulator protein in Saccharomyces cerevisiae.

O'Ryan L, Rimington T, Cant N, Ford RC.

J Vis Exp. 2012 Mar 10;(61). pii: 3860. doi: 10.3791/3860.

11.

Heterologous expression of human membrane receptors in the yeast Saccharomyces cerevisiae.

Joubert O, Nehmé R, Bidet M, Mus-Veteau I.

Methods Mol Biol. 2010;601:87-103. doi: 10.1007/978-1-60761-344-2_6.

PMID:
20099141
12.

Expression and purification of the mitochondrial uncoupling proteins: a comparative study between Escherichia coli and Saccharomyces cerevisiae.

Pecqueur C, Vacher D, Miroux B.

Biochem Soc Trans. 1999 Dec;27(6):888-93. Review. No abstract available.

PMID:
10830122
13.

Purification of TRAPP from Saccharomyces cerevisiae and identification of its mammalian counterpart.

Sacher M, Ferro-Novick S.

Methods Enzymol. 2001;329:234-41. No abstract available.

PMID:
11210539
14.
15.

A novel yeast expression system for the overproduction of quality-controlled membrane proteins.

Griffith DA, Delipala C, Leadsham J, Jarvis SM, Oesterhelt D.

FEBS Lett. 2003 Oct 9;553(1-2):45-50.

16.

A novel approach for the identification of protein-protein interaction with integral membrane proteins.

Hubsman M, Yudkovsky G, Aronheim A.

Nucleic Acids Res. 2001 Feb 15;29(4):E18.

17.

Protein expression-yeast.

Nielsen KH.

Methods Enzymol. 2014;536:133-47. doi: 10.1016/B978-0-12-420070-8.00012-X.

PMID:
24423273
18.

Purification and nucleic-acid-binding properties of a Saccharomyces cerevisiae protein involved in the control of ploidy.

Weber V, Wernitznig A, Hager G, Harata M, Frank P, Wintersberger U.

Eur J Biochem. 1997 Oct 1;249(1):309-17.

20.

Unconventional genomic architecture in the budding yeast saccharomyces cerevisiae masks the nested antisense gene NAG1.

Ma J, Dobry CJ, Krysan DJ, Kumar A.

Eukaryot Cell. 2008 Aug;7(8):1289-98. doi: 10.1128/EC.00053-08. Epub 2008 Feb 29.

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