Format
Sort by
Items per page

Send to

Choose Destination

Links from PubMed

Items: 1 to 20 of 173

1.

Recombinant protein expression by targeting pre-selected chromosomal loci.

Nehlsen K, Schucht R, da Gama-Norton L, Krömer W, Baer A, Cayli A, Hauser H, Wirth D.

BMC Biotechnol. 2009 Dec 14;9:100. doi: 10.1186/1472-6750-9-100.

2.
3.

[Development of site-specific integration system to high-level expression recombinant proteins in CHO cells].

Zhou H, Liu ZG, Sun ZW, Yu WY.

Sheng Wu Gong Cheng Xue Bao. 2007 Jul;23(4):756-62. Chinese.

PMID:
17822058
4.

An efficient and targeted gene integration system for high-level antibody expression.

Huang Y, Li Y, Wang YG, Gu X, Wang Y, Shen BF.

J Immunol Methods. 2007 Apr 30;322(1-2):28-39. Epub 2007 Feb 26.

PMID:
17350648
5.

A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors.

Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D.

Mol Ther. 2006 Aug;14(2):285-92. Epub 2006 May 11.

6.

Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.

Zhou H, Liu ZG, Sun ZW, Huang Y, Yu WY.

J Biotechnol. 2010 May 17;147(2):122-9. doi: 10.1016/j.jbiotec.2010.03.020. Epub 2010 Apr 3.

PMID:
20371256
7.

A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells.

Baser B, Spehr J, Büssow K, van den Heuvel J.

Methods. 2016 Feb 15;95:3-12. doi: 10.1016/j.ymeth.2015.11.022. Epub 2015 Dec 2.

8.

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

Chenuet S, Martinet D, Besuchet-Schmutz N, Wicht M, Jaccard N, Bon AC, Derouazi M, Hacker DL, Beckmann JS, Wurm FM.

Biotechnol Bioeng. 2008 Dec 1;101(5):937-45. doi: 10.1002/bit.21972.

PMID:
18781700
9.

Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange.

Feng YQ, Seibler J, Alami R, Eisen A, Westerman KA, Leboulch P, Fiering S, Bouhassira EE.

J Mol Biol. 1999 Oct 1;292(4):779-85.

PMID:
10525404
10.

Accumulative gene integration into a pre-determined site using Cre/loxP.

Obayashi H, Kawabe Y, Makitsubo H, Watanabe R, Kameyama Y, Huang S, Takenouchi Y, Ito A, Kamihira M.

J Biosci Bioeng. 2012 Mar;113(3):381-8. doi: 10.1016/j.jbiosc.2011.10.027. Epub 2011 Dec 1.

PMID:
22136795
11.

A modified RMCE-compatible Rosa26 locus for the expression of transgenes from exogenous promoters.

Tchorz JS, Suply T, Ksiazek I, Giachino C, Cloëtta D, Danzer CP, Doll T, Isken A, Lemaistre M, Taylor V, Bettler B, Kinzel B, Mueller M.

PLoS One. 2012;7(1):e30011. doi: 10.1371/journal.pone.0030011. Epub 2012 Jan 13.

12.

A novel Bxb1 integrase RMCE system for high fidelity site-specific integration of mAb expression cassette in CHO Cells.

Inniss MC, Bandara K, Jusiak B, Lu TK, Weiss R, Wroblewska L, Zhang L.

Biotechnol Bioeng. 2017 Aug;114(8):1837-1846. doi: 10.1002/bit.26268. Epub 2017 Mar 14.

PMID:
28186334
13.

Recombinase-mediated cassette exchange (RMCE) - a rapidly-expanding toolbox for targeted genomic modifications.

Turan S, Zehe C, Kuehle J, Qiao J, Bode J.

Gene. 2013 Feb 15;515(1):1-27. doi: 10.1016/j.gene.2012.11.016. Epub 2012 Nov 29. Review. Erratum in: Gene. 2013 May 10;520(1):77.

PMID:
23201421
14.

Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

Mayrhofer P, Kratzer B, Sommeregger W, Steinfellner W, Reinhart D, Mader A, Turan S, Qiao J, Bode J, Kunert R.

Appl Microbiol Biotechnol. 2014 Dec;98(23):9723-33. doi: 10.1007/s00253-014-6011-1. Epub 2014 Aug 27.

15.

A comparative study of different vector designs for the mammalian expression of recombinant IgG antibodies.

Li J, Menzel C, Meier D, Zhang C, Dübel S, Jostock T.

J Immunol Methods. 2007 Jan 10;318(1-2):113-24. Epub 2006 Nov 13.

PMID:
17161420
16.
17.

Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties.

Qiao J, Oumard A, Wegloehner W, Bode J.

J Mol Biol. 2009 Jul 24;390(4):579-94. doi: 10.1016/j.jmb.2009.05.012. Epub 2009 May 15.

PMID:
19447116
18.

Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site.

Kawabe Y, Inao T, Komatsu S, Huang G, Ito A, Omasa T, Kamihira M.

J Biosci Bioeng. 2017 Mar;123(3):390-397. doi: 10.1016/j.jbiosc.2016.10.011. Epub 2016 Nov 14.

PMID:
27856232
19.

Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methylation.

Spencer S, Gugliotta A, Koenitzer J, Hauser H, Wirth D.

J Biotechnol. 2015 Feb 10;195:15-29. doi: 10.1016/j.jbiotec.2014.12.009. Epub 2014 Dec 20.

PMID:
25533398
20.

Auditioning of CHO host cell lines using the artificial chromosome expression (ACE) technology.

Kennard ML, Goosney DL, Monteith D, Roe S, Fischer D, Mott J.

Biotechnol Bioeng. 2009 Oct 15;104(3):526-39. doi: 10.1002/bit.22407.

PMID:
19544304

Supplemental Content

Support Center