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Items: 1 to 20 of 211

1.

Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties.

Qiao J, Oumard A, Wegloehner W, Bode J.

J Mol Biol. 2009 Jul 24;390(4):579-94. doi: 10.1016/j.jmb.2009.05.012. Epub 2009 May 15.

PMID:
19447116
2.
3.

Recombinase-mediated cassette exchange (RMCE) - a rapidly-expanding toolbox for targeted genomic modifications.

Turan S, Zehe C, Kuehle J, Qiao J, Bode J.

Gene. 2013 Feb 15;515(1):1-27. doi: 10.1016/j.gene.2012.11.016. Epub 2012 Nov 29. Review. Erratum in: Gene. 2013 May 10;520(1):77.

PMID:
23201421
4.

Recombinase-mediated cassette exchange (RMCE): traditional concepts and current challenges.

Turan S, Galla M, Ernst E, Qiao J, Voelkel C, Schiedlmeier B, Zehe C, Bode J.

J Mol Biol. 2011 Mar 25;407(2):193-221. doi: 10.1016/j.jmb.2011.01.004. Epub 2011 Jan 15. Review.

PMID:
21241707
5.

Site-specific modification of the bovine genome using Cre recombinase-mediated gene targeting.

Graham C, Cole S, Laible G.

Biotechnol J. 2009 Jan;4(1):108-18. doi: 10.1002/biot.200800200.

PMID:
19156732
6.

Generation of a series of knock-in alleles using RMCE in ES cells.

Roebroek AJ, Gordts PL, Reekmans S.

Methods Mol Biol. 2011;693:277-81. doi: 10.1007/978-1-60761-974-1_16.

PMID:
21080286
7.

Selection-marker-free modification of the murine beta-casein gene using a lox2272 [correction of lox2722] site.

Kolb AF.

Anal Biochem. 2001 Mar;290(2):260-71. Erratum in: Anal Biochem 2001 Aug 1;295(1):127.

PMID:
11237328
8.

A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells.

Baser B, Spehr J, Büssow K, van den Heuvel J.

Methods. 2016 Feb 15;95:3-12. doi: 10.1016/j.ymeth.2015.11.022. Epub 2015 Dec 2.

9.

Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange.

Feng YQ, Seibler J, Alami R, Eisen A, Westerman KA, Leboulch P, Fiering S, Bouhassira EE.

J Mol Biol. 1999 Oct 1;292(4):779-85.

PMID:
10525404
10.
11.

The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes.

Bode J, Schlake T, Iber M, Schübeler D, Seibler J, Snezhkov E, Nikolaev L.

Biol Chem. 2000 Sep-Oct;381(9-10):801-13. Review.

PMID:
11076013
12.

[The study of a HPRT locus-specific transgenic method based on FLP recombinase mediated cassette exchange].

Zheng JM, He Y, Fu JL.

Shi Yan Sheng Wu Xue Bao. 2001 Dec;34(4):279-82. Chinese.

PMID:
12549206
13.

[Development of site-specific integration system to high-level expression recombinant proteins in CHO cells].

Zhou H, Liu ZG, Sun ZW, Yu WY.

Sheng Wu Gong Cheng Xue Bao. 2007 Jul;23(4):756-62. Chinese.

PMID:
17822058
14.

Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange.

Cesari F, Rennekampff V, Vintersten K, Vuong LG, Seibler J, Bode J, Wiebel FF, Nordheim A.

Genesis. 2004 Feb;38(2):87-92.

PMID:
14994271
16.

Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.

Lauth M, Spreafico F, Dethleffsen K, Meyer M.

Nucleic Acids Res. 2002 Nov 1;30(21):e115.

17.

Recombinant protein expression by targeting pre-selected chromosomal loci.

Nehlsen K, Schucht R, da Gama-Norton L, Krömer W, Baer A, Cayli A, Hauser H, Wirth D.

BMC Biotechnol. 2009 Dec 14;9:100. doi: 10.1186/1472-6750-9-100.

18.

Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange.

Baumann M, Gludovacz E, Sealover N, Bahr S, George H, Lin N, Kayser K, Borth N.

Biotechnol Bioeng. 2017 Nov;114(11):2616-2627. doi: 10.1002/bit.26388. Epub 2017 Aug 17.

PMID:
28734047
19.
20.

Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.

Zhou H, Liu ZG, Sun ZW, Huang Y, Yu WY.

J Biotechnol. 2010 May 17;147(2):122-9. doi: 10.1016/j.jbiotec.2010.03.020. Epub 2010 Apr 3.

PMID:
20371256

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