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Items: 1 to 20 of 133

1.

Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.

Li J, Wang L, Jänne PA, Makrigiorgos GM.

Clin Chem. 2009 Apr;55(4):748-56. doi: 10.1373/clinchem.2008.113381. Epub 2009 Feb 20.

2.

Temperature-tolerant COLD-PCR reduces temperature stringency and enables robust mutation enrichment.

Castellanos-Rizaldos E, Liu P, Milbury CA, Guha M, Brisci A, Cremonesi L, Ferrari M, Mamon H, Makrigiorgos GM.

Clin Chem. 2012 Jul;58(7):1130-8. doi: 10.1373/clinchem.2012.183095. Epub 2012 May 15.

3.

COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations.

Milbury CA, Li J, Makrigiorgos GM.

Clin Chem. 2009 Dec;55(12):2130-43. doi: 10.1373/clinchem.2009.131029. Epub 2009 Oct 8.

4.

[Detection of epidermal growth factor receptor mutations in non-small cell lung cancer by real-time PCR using TaqMan-MGB probes].

Zhou CC, Zhou SW, Pan H, Su B, Gao ZQ.

Zhonghua Zhong Liu Za Zhi. 2007 Feb;29(2):119-23. Chinese.

PMID:
17645848
5.

DMSO Increases Mutation Scanning Detection Sensitivity of High-Resolution Melting in Clinical Samples.

Song C, Castellanos-Rizaldos E, Bejar R, Ebert BL, Makrigiorgos GM.

Clin Chem. 2015 Nov;61(11):1354-62. doi: 10.1373/clinchem.2015.245357. Epub 2015 Oct 2.

6.

COLD-PCR enrichment of rare cancer mutations prior to targeted amplicon resequencing.

Milbury CA, Correll M, Quackenbush J, Rubio R, Makrigiorgos GM.

Clin Chem. 2012 Mar;58(3):580-9. doi: 10.1373/clinchem.2011.176198. Epub 2011 Dec 21.

7.
8.

Enrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.

Castellanos-Rizaldos E, Milbury CA, Makrigiorgos GM.

PLoS One. 2012;7(12):e51362. doi: 10.1371/journal.pone.0051362. Epub 2012 Dec 6.

9.

Enhanced ratio of signals enables digital mutation scanning for rare allele detection.

Castellanos-Rizaldos E, Paweletz C, Song C, Oxnard GR, Mamon H, Jänne PA, Makrigiorgos GM.

J Mol Diagn. 2015 May;17(3):284-92. doi: 10.1016/j.jmoldx.2014.12.003. Epub 2015 Mar 13.

10.
11.

COLD-PCR: improving the sensitivity of molecular diagnostics assays.

Milbury CA, Li J, Liu P, Makrigiorgos GM.

Expert Rev Mol Diagn. 2011 Mar;11(2):159-69. doi: 10.1586/erm.10.115.

12.

The "COLD-PCR approach" for early and cost-effective detection of tyrosine kinase inhibitor resistance mutations in EGFR-positive non-small cell lung cancer.

Mairinger FD, Vollbrecht C, Streubel A, Roth A, Landt O, Walter HF, Kollmeier J, Mairinger T.

Appl Immunohistochem Mol Morphol. 2014;22(2):114-8. doi: 10.1097/PDM.0b013e31829a638d.

PMID:
24517913
13.

Detection of hepatitis B virus genotypic resistance mutations by coamplification at lower denaturation temperature-PCR coupled with sanger sequencing.

Liu C, Lin J, Chen H, Shang H, Jiang L, Chen J, Ye Y, Yang B, Ou Q.

J Clin Microbiol. 2014 Aug;52(8):2933-9. doi: 10.1128/JCM.01127-14. Epub 2014 Jun 4.

14.

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.

Li J, Makrigiorgos GM.

Biochem Soc Trans. 2009 Apr;37(Pt 2):427-32. doi: 10.1042/BST0370427. Review.

PMID:
19290875
15.

Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products.

Kristensen LS, Daugaard IL, Christensen M, Hamilton-Dutoit S, Hager H, Hansen LL.

Hum Mutat. 2010 Dec;31(12):1366-73. doi: 10.1002/humu.21358. Epub 2010 Oct 26.

PMID:
20848649
16.

Application of coamplification at lower denaturation temperature-PCR sequencing for early detection of antiviral drug resistance mutations of hepatitis B virus.

Wong DK, Tsoi O, Huang FY, Seto WK, Fung J, Lai CL, Yuen MF.

J Clin Microbiol. 2014 Sep;52(9):3209-15. doi: 10.1128/JCM.00343-14. Epub 2014 Jun 20.

17.

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.

Li J, Wang L, Mamon H, Kulke MH, Berbeco R, Makrigiorgos GM.

Nat Med. 2008 May;14(5):579-84. doi: 10.1038/nm1708. Epub 2008 Apr 13.

PMID:
18408729
18.

Coamplification at lower denaturation temperature polymerase chain reaction enables selective identification of K-Ras mutations in formalin-fixed, paraffin-embedded tumor tissues without tumor-cell enrichment.

Yu S, Xie L, Hou Z, Qian X, Yu L, Wei J, Ding Y, Liu B.

Hum Pathol. 2011 Sep;42(9):1312-8. doi: 10.1016/j.humpath.2010.06.018. Epub 2011 Mar 15.

PMID:
21406308
20.

PCR-based methods for the enrichment of minority alleles and mutations.

Milbury CA, Li J, Makrigiorgos GM.

Clin Chem. 2009 Apr;55(4):632-40. doi: 10.1373/clinchem.2008.113035. Epub 2009 Feb 6. Review.

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