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Items: 1 to 20 of 111

1.

Evolution of a fluorinated green fluorescent protein.

Yoo TH, Link AJ, Tirrell DA.

Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13887-90. Epub 2007 Aug 23.

2.

Experimental evolution of a green fluorescent protein composed of 19 unique amino acids without tryptophan.

Kawahara-Kobayashi A, Hitotsuyanagi M, Amikura K, Kiga D.

Orig Life Evol Biosph. 2014 Apr;44(2):75-86. doi: 10.1007/s11084-014-9371-8. Epub 2014 Nov 16.

PMID:
25399308
3.

Engineering and characterization of a superfolder green fluorescent protein.

Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS.

Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170.

PMID:
16369541
4.

Improved green fluorescent protein by molecular evolution using DNA shuffling.

Crameri A, Whitehorn EA, Tate E, Stemmer WP.

Nat Biotechnol. 1996 Mar;14(3):315-9.

PMID:
9630892
5.

Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening.

Seitz T, Thoma R, Schoch GA, Stihle M, Benz J, D'Arcy B, Wiget A, Ruf A, Hennig M, Sterner R.

J Mol Biol. 2010 Nov 5;403(4):562-77. doi: 10.1016/j.jmb.2010.08.048. Epub 2010 Sep 17.

PMID:
20850457
6.

Improved solubility of TEV protease by directed evolution.

van den Berg S, Löfdahl PA, Härd T, Berglund H.

J Biotechnol. 2006 Feb 10;121(3):291-8. Epub 2005 Sep 15.

PMID:
16150509
7.

Engineering color variants of green fluorescent protein (GFP) for thermostability, pH-sensitivity, and improved folding kinetics.

Aliye N, Fabbretti A, Lupidi G, Tsekoa T, Spurio R.

Appl Microbiol Biotechnol. 2015 Feb;99(3):1205-16. doi: 10.1007/s00253-014-5975-1. Epub 2014 Aug 13.

PMID:
25112226
8.

Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells.

Kremers GJ, Goedhart J, van den Heuvel DJ, Gerritsen HC, Gadella TW Jr.

Biochemistry. 2007 Mar 27;46(12):3775-83. Epub 2007 Feb 27.

PMID:
17323929
9.

Directed evolution study unveiling key sequence factors that affect translation efficiency in Escherichia coli.

Tsukuda M, Miyazaki K.

J Biosci Bioeng. 2013 Nov;116(5):540-5. doi: 10.1016/j.jbiosc.2013.05.013. Epub 2013 Jun 18.

PMID:
23790548
10.

Cd-specific mutants of mercury-sensing regulatory protein MerR, generated by directed evolution.

Hakkila KM, Nikander PA, Junttila SM, Lamminmäki UJ, Virta MP.

Appl Environ Microbiol. 2011 Sep;77(17):6215-24. doi: 10.1128/AEM.00662-11. Epub 2011 Jul 15.

11.

A practical teaching course in directed protein evolution using the green fluorescent protein as a model.

Ruller R, Silva-Rocha R, Silva A, Cruz Schneider MP, Ward RJ.

Biochem Mol Biol Educ. 2011 Jan-Feb;39(1):21-7. doi: 10.1002/bmb.20430.

12.

FACS-optimized mutants of the green fluorescent protein (GFP).

Cormack BP, Valdivia RH, Falkow S.

Gene. 1996;173(1 Spec No):33-8.

PMID:
8707053
13.

Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations.

Liu SS, Wei X, Dong X, Xu L, Liu J, Jiang B.

BMC Biochem. 2015 Jul 25;16:17. doi: 10.1186/s12858-015-0046-5.

14.

Rapid protein-folding assay using green fluorescent protein.

Waldo GS, Standish BM, Berendzen J, Terwilliger TC.

Nat Biotechnol. 1999 Jul;17(7):691-5.

PMID:
10404163
15.

Facilitating chromophore formation of engineered Ca(2+) binding green fluorescent proteins.

Holder AN, Ellis AL, Zou J, Chen N, Yang JJ.

Arch Biochem Biophys. 2009 Jun 1;486(1):27-34. doi: 10.1016/j.abb.2009.03.016. Epub 2009 Apr 7.

16.

A fusion tag to fold on: the S-layer protein SgsE confers improved folding kinetics to translationally fused enhanced green fluorescent protein.

Ristl R, Kainz B, Stadlmayr G, Schuster H, Pum D, Messner P, Obinger C, Schaffer C.

J Microbiol Biotechnol. 2012 Sep;22(9):1271-8.

17.

Random single amino acid deletion sampling unveils structural tolerance and the benefits of helical registry shift on GFP folding and structure.

Arpino JA, Reddington SC, Halliwell LM, Rizkallah PJ, Jones DD.

Structure. 2014 Jun 10;22(6):889-98. doi: 10.1016/j.str.2014.03.014. Epub 2014 May 22.

18.

Redesigning the type II' β-turn in green fluorescent protein to type I': implications for folding kinetics and stability.

Madan B, Sokalingam S, Raghunathan G, Lee SG.

Proteins. 2014 Oct;82(10):2812-22. doi: 10.1002/prot.24644. Epub 2014 Jul 31.

PMID:
25044033
19.
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