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A microchip approach for practical label-free CD4+ T-cell counting of HIV-infected subjects in resource-poor settings.

Cheng X, Irimia D, Dixon M, Ziperstein JC, Demirci U, Zamir L, Tompkins RG, Toner M, Rodriguez WR.

J Acquir Immune Defic Syndr. 2007 Jul 1;45(3):257-61.


A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects.

Cheng X, Irimia D, Dixon M, Sekine K, Demirci U, Zamir L, Tompkins RG, Rodriguez W, Toner M.

Lab Chip. 2007 Feb;7(2):170-8.


Enhancing the performance of a point-of-care CD4+ T-cell counting microchip through monocyte depletion for HIV/AIDS diagnostics.

Cheng X, Gupta A, Chen C, Tompkins RG, Rodriguez W, Toner M.

Lab Chip. 2009 May 21;9(10):1357-64. doi: 10.1039/b818813k.


Enumeration of CD4+ T-cells using a portable microchip count platform in Tanzanian HIV-infected patients.

Moon S, Gurkan UA, Blander J, Fawzi WW, Aboud S, Mugusi F, Kuritzkes DR, Demirci U.

PLoS One. 2011;6(7):e21409. doi: 10.1371/journal.pone.0021409.


Affordable CD4+ T cell counts by flow cytometry. II. The use of fixed whole blood in resource-poor settings.

Jani V, Janossy G, Iqbal A, Mhalu FS, Lyamuya EF, Biberfeld G, Glencross DK, Scott L, Reilly JT, Granger V, Barnett D.

J Immunol Methods. 2001 Nov 1;257(1-2):145-54.


A microchip CD4 counting method for HIV monitoring in resource-poor settings.

Rodriguez WR, Christodoulides N, Floriano PN, Graham S, Mohanty S, Dixon M, Hsiang M, Peter T, Zavahir S, Thior I, Romanovicz D, Bernard B, Goodey AP, Walker BD, McDevitt JT.

PLoS Med. 2005 Jul;2(7):e182.


Microfluidic CD4+ T-cell counting device using chemiluminescence-based detection.

Wang Z, Chin SY, Chin CD, Sarik J, Harper M, Justman J, Sia SK.

Anal Chem. 2010 Jan 1;82(1):36-40. doi: 10.1021/ac902144w.


Comparison of CD4 cell count by a simple enzyme-linked immunosorbent assay using the TRAx CD4 test kit and by flow cytometry and hematology.

Paxton H, Pins M, Denton G, McGonigle AD, Meisner PS, Phair JP.

Clin Diagn Lab Immunol. 1995 Jan;2(1):104-14.


The use of glutaraldehyde-fixed chicken red blood cells as counting beads for performing affordable single-platform CD4(+) T-lymphocyte count in HIV-1-infected patients.

Pattanapanyasat K, Noulsri E, Lerdwana S, Sukapirom K, Onlamoon N, Tassaneetrithep B.

J Acquir Immune Defic Syndr. 2010 Jan;53(1):47-54. doi: 10.1097/QAI.0b013e3181c4b8ae.


A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings.

Balakrishnan P, Solomon S, Mohanakrishnan J, Cecelia AJ, Kumarasamy N, Murugavel KG, Venkatakrishnan B, Solomon SS, Crowe SM, Ganesh AK, Thyagarajan SP, Flanigan T, Mayer KH.

J Acquir Immune Defic Syndr. 2006 Sep;43(1):23-6.


Magnetic bead based immunoassay for enumeration of CD4+ T lymphocytes on a microfluidic device.

Gao D, Li HF, Guo GS, Lin JM.

Talanta. 2010 Jul 15;82(2):528-33. doi: 10.1016/j.talanta.2010.05.019.


Rapid automated cell quantification on HIV microfluidic devices.

Alyassin MA, Moon S, Keles HO, Manzur F, Lin RL, Hæggstrom E, Kuritzkes DR, Demirci U.

Lab Chip. 2009 Dec 7;9(23):3364-9. doi: 10.1039/b911882a.


Microfluidic CD4+ and CD8+ T lymphocyte counters for point-of-care HIV diagnostics using whole blood.

Watkins NN, Hassan U, Damhorst G, Ni H, Vaid A, Rodriguez W, Bashir R.

Sci Transl Med. 2013 Dec 4;5(214):214ra170. doi: 10.1126/scitranslmed.3006870.


Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings.

Koyalta D, Jenabian MA, Nadjiouroum N, Djouater B, Djemadji-Oudjeil N, Ndjoyi-Mbiguino A, Bélec L.

BMC Res Notes. 2013 Apr 30;6:169. doi: 10.1186/1756-0500-6-169.


Absolute CD4 T-cell counting in resource-poor settings: direct volumetric measurements versus bead-based clinical flow cytometry instruments.

Dieye TN, Vereecken C, Diallo AA, Ondoa P, Diaw PA, Camara M, Karam F, Mboup S, Kestens L.

J Acquir Immune Defic Syndr. 2005 May 1;39(1):32-7.


Evaluation of a new single-parameter volumetric flow cytometer (CyFlow(green)) for enumeration of absolute CD4+ T lymphocytes in human immunodeficiency virus type 1-infected Thai patients.

Pattanapanyasat K, Lerdwana S, Noulsri E, Chaowanachan T, Wasinrapee P, Sakulploy N, Pobkeeree V, Suksripanich O, Thanprasertsuk S, Spira TJ, Tappero JW, Levine WC.

Clin Diagn Lab Immunol. 2005 Dec;12(12):1416-24.


Enumeration of the absolute CD4 T-lymphocyte count by cell-bead assay.

Nantakomol D, Nuchnoi P, Noulsri E, Lerdwana S, Krisin S, Chanprasert S, Pattanapanyasat K.

Cytometry B Clin Cytom. 2010 Jul;78(4):260-6. doi: 10.1002/cyto.b.20518.


Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices.

Cheng X, Liu YS, Irimia D, Demirci U, Yang L, Zamir L, Rodríguez WR, Toner M, Bashir R.

Lab Chip. 2007 Jun;7(6):746-55.


Low-cost CD4 enumeration in HIV-infected patients in Thailand.

Pattanapanyasat K, Lerdwana S, Shain H, Noulsri E, Thepthai C, Prasertsilpa V, Eksaengsri A, Kraisintu K.

Asian Pac J Allergy Immunol. 2003 Jun;21(2):105-13.


On-chip sample preparation by controlled release of antibodies for simple CD4 counting.

Beck M, Brockhuis S, van der Velde N, Breukers C, Greve J, Terstappen LW.

Lab Chip. 2012 Jan 7;12(1):167-73. doi: 10.1039/c1lc20565j.

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