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Items: 1 to 20 of 94

1.

Second-generation shRNA libraries covering the mouse and human genomes.

Silva JM, Li MZ, Chang K, Ge W, Golding MC, Rickles RJ, Siolas D, Hu G, Paddison PJ, Schlabach MR, Sheth N, Bradshaw J, Burchard J, Kulkarni A, Cavet G, Sachidanandam R, McCombie WR, Cleary MA, Elledge SJ, Hannon GJ.

Nat Genet. 2005 Nov;37(11):1281-8.

PMID:
16200065
2.

Lessons from Nature: microRNA-based shRNA libraries.

Chang K, Elledge SJ, Hannon GJ.

Nat Methods. 2006 Sep;3(9):707-14. Review.

PMID:
16929316
3.

An arrayed genome-scale lentiviral-enabled short hairpin RNA screen identifies lethal and rescuer gene candidates.

Bhinder B, Antczak C, Ramirez CN, Shum D, Liu-Sullivan N, Radu C, Frattini MG, Djaballah H.

Assay Drug Dev Technol. 2013 Apr;11(3):173-90. doi: 10.1089/adt.2012.475.

4.

Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cells.

Cheng TL, Chang WT.

Methods Mol Biol. 2007;408:223-41. doi: 10.1007/978-1-59745-547-3_13.

PMID:
18314586
5.

A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen.

Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE.

Cell. 2006 Mar 24;124(6):1283-98.

6.

Next-generation libraries for robust RNA interference-based genome-wide screens.

Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS.

Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3384-91. doi: 10.1073/pnas.1508821112.

7.

A primer on using pooled shRNA libraries for functional genomic screens.

Hu G, Luo J.

Acta Biochim Biophys Sin (Shanghai). 2012 Feb;44(2):103-12. doi: 10.1093/abbs/gmr116. Review.

8.

High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing.

Sims D, Mendes-Pereira AM, Frankum J, Burgess D, Cerone MA, Lombardelli C, Mitsopoulos C, Hakas J, Murugaesu N, Isacke CM, Fenwick K, Assiotis I, Kozarewa I, Zvelebil M, Ashworth A, Lord CJ.

Genome Biol. 2011 Oct 21;12(10):R104. doi: 10.1186/gb-2011-12-10-r104.

9.

A resource for large-scale RNA-interference-based screens in mammals.

Paddison PJ, Silva JM, Conklin DS, Schlabach M, Li M, Aruleba S, Balija V, O'Shaughnessy A, Gnoj L, Scobie K, Chang K, Westbrook T, Cleary M, Sachidanandam R, McCombie WR, Elledge SJ, Hannon GJ.

Nature. 2004 Mar 25;428(6981):427-31.

PMID:
15042091
10.

Pooled shRNA screenings: experimental approach.

Rodriguez-Barrueco R, Marshall N, Silva JM.

Methods Mol Biol. 2013;980:353-70. doi: 10.1007/978-1-62703-287-2_21.

PMID:
23359166
11.

Enzymatic production of RNAi libraries from cDNAs.

Shirane D, Sugao K, Namiki S, Tanabe M, Iino M, Hirose K.

Nat Genet. 2004 Feb;36(2):190-6.

12.

A shRNA library constructed through the generation of loop-stem-loop DNA.

Nishikawa Y, Sugiyama T.

J Gene Med. 2010 Nov;12(11):927-33. doi: 10.1002/jgm.1513.

PMID:
21105152
13.

Enzymatically prepared RNAi libraries.

Buchholz F, Kittler R, Slabicki M, Theis M.

Nat Methods. 2006 Sep;3(9):696-700. Review.

PMID:
16929314
14.

shRNA libraries and their use in cancer genetics.

Bernards R, Brummelkamp TR, Beijersbergen RL.

Nat Methods. 2006 Sep;3(9):701-6. Review.

PMID:
16929315
15.

Enzymatic production of RNAi libraries from cDNAs and high-throughput selection of effective shRNA expression constructs.

Sugao K, Hirose K.

Methods Mol Biol. 2011;729:123-39. doi: 10.1007/978-1-61779-065-2_8.

PMID:
21365487
16.

Construction of equalized short hairpin RNA library from human brain cDNA.

Xu L, Li J, Liu L, Lu L, Gao J, Li X.

J Biotechnol. 2007 Feb 20;128(3):477-85.

PMID:
17187888
17.

RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age.

Silva J, Chang K, Hannon GJ, Rivas FV.

Oncogene. 2004 Nov 1;23(51):8401-9. Review.

PMID:
15517022
18.

Functional selection of shRNA loops from randomized retroviral libraries.

Jensen SM, Schmitz A, Pedersen FS, Kjems J, Bramsen JB.

PLoS One. 2012;7(8):e43095. doi: 10.1371/journal.pone.0043095.

19.

Genome-wide application of RNAi to the discovery of potential drug targets.

Ito M, Kawano K, Miyagishi M, Taira K.

FEBS Lett. 2005 Oct 31;579(26):5988-95. Review.

20.

RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs.

Olson A, Sheth N, Lee JS, Hannon G, Sachidanandam R.

Nucleic Acids Res. 2006 Jan 1;34(Database issue):D153-7.

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