Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630

Thomas Riedel; Boyke Bunk; Andrea Thürmer; Cathrin Spröer; Elzbieta Brzuszkiewicz; Birte Abt; Sabine Gronow; Heiko Liesegang; Rolf Daniel; Jörg Overmann

doi:10.1128/genomeA.00276-15

Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630

Genome Announc. 2015 Apr 9;3(2):e00276-15. doi: 10.1128/genomeA.00276-15.

Authors

Thomas Riedel¹, Boyke Bunk, Andrea Thürmer², Cathrin Spröer³, Elzbieta Brzuszkiewicz², Birte Abt, Sabine Gronow, Heiko Liesegang², Rolf Daniel², Jörg Overmann

Affiliations

¹ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany thomas.riedel@dsmz.de.
² Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Göttingen, Germany.
³ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.

Abstract

We resequenced the complete genome of the virulent and multidrug-resistant pathogen Clostridium difficile strain 630. A combination of single-molecule real-time and Illumina sequencing technology revealed the presence of an additional rRNA gene cluster, additional tRNAs, and the absence of a transposon in comparison to the published and reannotated genome sequence.