Abstract
We report the isolation of a large cyclophilin protein containing RS (arginine-serine) repeats from a yeast two-hybrid screen using ClK (CDC28/cdc2-like kinase) as a probe. This Clk associating RS-cyclophilin (CARS-Cyp) possesses 39% homology to the NK-TR1 (natural killer tumor recognition protein-1) we have previously characterized (Anderson et al. (1993) Proc. Natl. Acad. Sci. USA 90 (1993) 542-546). CARS-Cyp is expressed in a variety of tissues and cell types, and codes for a protein with a predicted mass of 89 kDa containing a cyclophilin-related domain, two Nopp140 (nucleolar phosphoprotein of 140 kDa)-related domains, and a large RS domain. The RS-cyclophilins, a novel class of proteins, may play an important role in the regulation of pre-mRNA splicing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Isomerases / chemistry
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Amino Acid Isomerases / genetics
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Amino Acid Isomerases / isolation & purification*
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Amino Acid Sequence
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Animals
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Arginine / chemistry
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Base Sequence
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Blood Proteins / chemistry*
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification*
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Cloning, Molecular
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Cyclophilins*
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DNA, Complementary
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Gene Expression
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Humans
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Killer Cells, Natural / chemistry
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Mice
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Molecular Sequence Data
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Protein Serine-Threonine Kinases / metabolism
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Protein-Tyrosine Kinases / metabolism
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RNA Splicing
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Receptors, Natural Killer Cell
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Serine / chemistry
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T-Lymphocytes / chemistry
Substances
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Blood Proteins
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Carrier Proteins
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DNA, Complementary
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Receptors, Natural Killer Cell
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Serine
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Arginine
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Clk dual-specificity kinases
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Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
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Amino Acid Isomerases
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Cyclophilins
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PPIG protein, human