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J Gen Virol. 2009 Jun;90(Pt 6):1433-9. doi: 10.1099/vir.0.010074-0. Epub 2009 Mar 12.

Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia.

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1
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia. chris.cowled@csiro.au

Abstract

Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled Culex annulirostris mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus Orbivirus, family Reoviridae. Here, a cDNA subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner-core protein). Phylogenetic analysis showed that SLOV should be considered a new species within the genus Orbivirus. A real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.

PMID:
19282430
PMCID:
PMC2887561
DOI:
10.1099/vir.0.010074-0
[Indexed for MEDLINE]
Free PMC Article
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