Prevalence of fosfomycin resistance among CTX-M-producing Escherichia coli clinical isolates in Japan and identification of novel plasmid-mediated fosfomycin-modifying enzymes

Jun-ichi Wachino; Kunikazu Yamane; Satowa Suzuki; Kouji Kimura; Yoshichika Arakawa

doi:10.1128/AAC.01834-09

Prevalence of fosfomycin resistance among CTX-M-producing Escherichia coli clinical isolates in Japan and identification of novel plasmid-mediated fosfomycin-modifying enzymes

Antimicrob Agents Chemother. 2010 Jul;54(7):3061-4. doi: 10.1128/AAC.01834-09. Epub 2010 Apr 19.

Authors

Jun-ichi Wachino¹, Kunikazu Yamane, Satowa Suzuki, Kouji Kimura, Yoshichika Arakawa

Affiliation

¹ Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan. wachino@nih.go.jp

Abstract

We evaluated the in vitro activity of fosfomycin against a total of 192 CTX-M beta-lactamase-producing Escherichia coli strains isolated in 70 Japanese clinical settings. Most of the isolates (96.4%) were found to be susceptible to fosfomycin. On the other hand, some of the resistant isolates were confirmed to harbor the novel transferable fosfomycin resistance determinants named FosA3 and FosC2, which efficaciously inactivate fosfomycin through glutathione S-transferase activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Drug Resistance, Bacterial / genetics
Escherichia coli / drug effects*
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics
Fosfomycin / pharmacology*
Glutathione Transferase / genetics
Japan
Microbial Sensitivity Tests
Molecular Sequence Data
Plasmids / genetics*
Sequence Homology, Amino Acid
beta-Lactamases / metabolism*

Substances

Escherichia coli Proteins
FosA(3) protein, E coli
Fosfomycin
Glutathione Transferase
beta-Lactamases

Associated data

GENBANK/AB522969
GENBANK/AB522970