Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay

J Clin Microbiol. 2004 Apr;42(4):1723-6. doi: 10.1128/JCM.42.4.1723-1726.2004.

Abstract

Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Infections, Human / virology
  • Adenoviruses, Human / classification*
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / isolation & purification
  • Brazil
  • Child, Preschool
  • DNA, Viral / analysis
  • Diarrhea / virology*
  • Feces / virology
  • Genotype
  • Heteroduplex Analysis / methods*
  • Humans
  • Infant
  • Infant, Newborn
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational*
  • Restriction Mapping
  • Sequence Analysis, DNA

Substances

  • DNA, Viral

Associated data

  • GENBANK/AY375456
  • GENBANK/AY375457
  • GENBANK/AY375458
  • GENBANK/AY375459
  • GENBANK/AY375460
  • GENBANK/AY375461
  • GENBANK/AY375462