Format

Send to

Choose Destination

Links from Nucleotide

See comment in PubMed Commons below
Gene. 1993 May 30;127(2):233-6.

Construction of a retrotransposition indicator sequence using a neomycin resistance-encoding gene containing a functional intron.

Author information

1
Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston 29425.

Abstract

An intron from a Syrian hamster gene was inserted into a neo gene such that splicing of the neo gene mRNA results in the synthesis of active aminoglycoside phosphotransferase. The unspliced construct is inactive in Escherichia coli, but confers resistance to G418 when transfected into mouse and hamster cells. This selectable marker is designed to aid in the cloning and identification of genomic integration sites following retrotransposition.

PMID:
8388842
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center