Abstract
CpG islands are short stretches of DNA containing a high density of non-methylated CpG dinucleotides, predominantly associated with coding regions. We have constructed an affinity matrix that contains the methyl-CpG binding domain from the rat chromosomal protein MeCP2, attached to a solid support. A column containing the matrix fractionates DNA according to its degree of CpG methylation, strongly retaining those sequences that are highly methylated. Using this column, we have developed a procedure for bulk isolation of CpG islands from human genomic DNA. As CpG islands overlap with approximately 60% of human genes, the resulting CpG island library can be used to isolate full-length cDNAs and to place genes on genomic maps.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromatography, Affinity
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Cloning, Molecular
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DNA / chemistry
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DNA / genetics*
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DNA / isolation & purification*
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DNA Primers / genetics
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DNA-Binding Proteins / genetics
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Gene Library
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Humans
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Male
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Methylation
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Molecular Sequence Data
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Oligodeoxyribonucleotides / genetics*
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Oligodeoxyribonucleotides / isolation & purification*
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RNA, Ribosomal / genetics
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Rats
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Repetitive Sequences, Nucleic Acid
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Transcription, Genetic
Substances
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DNA Primers
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DNA-Binding Proteins
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Oligodeoxyribonucleotides
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RNA, Ribosomal
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DNA
Associated data
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GENBANK/X76662
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GENBANK/X76663
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GENBANK/X76664
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GENBANK/X76665
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GENBANK/X76666
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GENBANK/X76667
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GENBANK/X76668
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GENBANK/X76669
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GENBANK/X76670
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GENBANK/X76671
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GENBANK/X76672
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GENBANK/X76673
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GENBANK/X76674
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GENBANK/X76675
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GENBANK/X76676
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GENBANK/X76677
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GENBANK/X76694
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GENBANK/X76695