Abstract
Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2 approximately q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics*
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Chromosomes, Human, Pair 8
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Cysteine Endopeptidases / genetics*
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / genetics*
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Gene Amplification*
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Humans
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Mixed Tumor, Malignant / genetics*
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Mixed Tumor, Malignant / pathology
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Multienzyme Complexes / genetics*
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Nucleic Acid Hybridization
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Oligonucleotide Array Sequence Analysis
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Proteasome Endopeptidase Complex
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Reverse Transcriptase Polymerase Chain Reaction
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Salivary Gland Neoplasms / genetics*
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Salivary Gland Neoplasms / pathology
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src-Family Kinases / genetics*
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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Multienzyme Complexes
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PLAG1 protein, human
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lyn protein-tyrosine kinase
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src-Family Kinases
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Cysteine Endopeptidases
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Proteasome Endopeptidase Complex
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Adenosine Triphosphatases