Abstract
Mice deficient in 14-3-3epsilon exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3epsilon interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3epsilon and for maintaining USP8 in the cytosol.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
MeSH terms
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14-3-3 Proteins / analysis
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14-3-3 Proteins / metabolism*
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Amino Acid Sequence
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Animals
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Binding Sites
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Brain / embryology*
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Brain / metabolism*
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COS Cells
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Chlorocebus aethiops
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Endopeptidases / genetics
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Endopeptidases / metabolism*
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Endosomal Sorting Complexes Required for Transport
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Mass Spectrometry
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Mice
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Microscopy, Fluorescence
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Molecular Sequence Data
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Phosphorylation
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Ubiquitin Thiolesterase
Substances
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14-3-3 Proteins
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Endosomal Sorting Complexes Required for Transport
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Endopeptidases
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Ubiquitin Thiolesterase
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Usp8 protein, mouse
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ubiquitin isopeptidase