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Items: 1 to 20 of 94

1.

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron.

Taxis C, Stier G, Spadaccini R, Knop M.

Mol Syst Biol. 2009;5:267. doi: 10.1038/msb.2009.25.

2.

Targeted protein depletion in Saccharomyces cerevisiae by activation of a bidirectional degron.

Jungbluth M, Renicke C, Taxis C.

BMC Syst Biol. 2010 Dec 29;4:176. doi: 10.1186/1752-0509-4-176.

3.

TIPI: TEV protease-mediated induction of protein instability.

Taxis C, Knop M.

Methods Mol Biol. 2012;832:611-26. doi: 10.1007/978-1-61779-474-2_43.

PMID:
22350916
4.

Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW.

Sasabe M, Shintani S, Kintaka R, Kaizu K, Makanae K, Moriya H.

BMC Syst Biol. 2014 Jan 7;8:2. doi: 10.1186/1752-0509-8-2.

5.

Rapid depletion of budding yeast proteins by fusion to a heat-inducible degron.

Sanchez-Diaz A, Kanemaki M, Marchesi V, Labib K.

Sci STKE. 2004 Mar 2;2004(223):PL8.

PMID:
15010550
6.

Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo.

Kanemaki M, Sanchez-Diaz A, Gambus A, Labib K.

Nature. 2003 Jun 12;423(6941):720-4.

PMID:
12768207
7.

Blocking endocytotic mechanisms to improve heterologous protein titers in Saccharomyces cerevisiae.

Rodríguez-Limas WA, Tannenbaum V, Tyo KE.

Biotechnol Bioeng. 2015 Feb;112(2):376-85. doi: 10.1002/bit.25360.

PMID:
25154809
8.

Structural basis for the recognition of N-end rule substrates by the UBR box of ubiquitin ligases.

Choi WS, Jeong BC, Joo YJ, Lee MR, Kim J, Eck MJ, Song HK.

Nat Struct Mol Biol. 2010 Oct;17(10):1175-81. doi: 10.1038/nsmb.1907.

PMID:
20835240
9.

Control of protein quality and stoichiometries by N-terminal acetylation and the N-end rule pathway.

Shemorry A, Hwang CS, Varshavsky A.

Mol Cell. 2013 May 23;50(4):540-51. doi: 10.1016/j.molcel.2013.03.018.

10.

Heat-inducible degron and the making of conditional mutants.

Dohmen RJ, Varshavsky A.

Methods Enzymol. 2005;399:799-822.

PMID:
16338396
11.

An expanded tool kit for the auxin-inducible degron system in budding yeast.

Morawska M, Ulrich HD.

Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967.

12.

Analysis of a conditional degradation signal in yeast and mammalian cells.

Lévy F, Johnston JA, Varshavsky A.

Eur J Biochem. 1999 Jan;259(1-2):244-52.

13.

Heterologous expression studies of Saccharomyces cerevisiae reveal two distinct trypanosomatid CaaX protease activities and identify their potential targets.

Mokry DZ, Manandhar SP, Chicola KA, Santangelo GM, Schmidt WK.

Eukaryot Cell. 2009 Dec;8(12):1891-900. doi: 10.1128/EC.00169-09.

14.

An auxin-based degron system for the rapid depletion of proteins in nonplant cells.

Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M.

Nat Methods. 2009 Dec;6(12):917-22. doi: 10.1038/nmeth.1401.

PMID:
19915560
15.

Inducible degron and its application to creating conditional mutants.

Dohmen RJ.

Methods Mol Biol. 2006;313:145-59.

PMID:
16118432
16.

TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms.

Harder B, Schomburg A, Pflanz R, Küstner K, Gerlach N, Schuh R.

Biotechniques. 2008 May;44(6):765-72. doi: 10.2144/000112884.

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19.

The prodomain of Ssy5 protease controls receptor-activated proteolysis of transcription factor Stp1.

Pfirrmann T, Heessen S, Omnus DJ, Andréasson C, Ljungdahl PO.

Mol Cell Biol. 2010 Jul;30(13):3299-309. doi: 10.1128/MCB.00323-10.

20.
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