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Dev Cell. 2018 May 7;45(3):406-416.e3. doi: 10.1016/j.devcel.2018.03.012. Epub 2018 Apr 12.

DNA N6-Adenine Methylation in Arabidopsis thaliana.

Author information

1
Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
2
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
3
Nextomics Biosciences, Wuhan 430075, China.
4
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: guxiaofeng@caas.cn.
5
Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore. Electronic address: dbsyuhao@nus.edu.sg.

Abstract

DNA methylation on N6-adenine (6mA) has recently been found to be a potentially epigenetic mark in several unicellular and multicellular eukaryotes. However, its distribution patterns and potential functions in land plants, which are primary producers for most ecosystems, remain largely unknown. Here we report global profiling of 6mA sites at single-nucleotide resolution in the genome of Arabidopsis thaliana at different developmental stages using single-molecule real-time sequencing. 6mA sites are widely distributed across the Arabidopsis genome and enriched over the pericentromeric heterochromatin regions. 6mA occurs more frequently in gene bodies than intergenic regions. Analysis of 6mA methylomes and RNA sequencing data demonstrates that 6mA frequency positively correlates with the gene expression level and the transition from vegetative to reproductive growth in Arabidopsis. Our results uncover 6mA as a DNA mark associated with actively expressed genes in Arabidopsis, suggesting that 6mA serves as a hitherto unknown epigenetic mark in land plants.

KEYWORDS:

6mA; Arabidopsis thaliana; DNA methylation; N(6)-adenine; epigenetic mark; methylome; transcription

PMID:
29656930
DOI:
10.1016/j.devcel.2018.03.012
[Indexed for MEDLINE]
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