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Avian Pathol. 2017 Oct;46(5):541-547. doi: 10.1080/03079457.2017.1324198. Epub 2017 Jun 5.

Contribution of the csgA and bcsA genes to Salmonella enterica serovar Pullorum biofilm formation and virulence.

El Hag M1,2,3,4, Feng Z1,2,3, Su Y1,2,3, Wang X1,2,3, Yassin A2,4, Chen S1,2,3, Peng D1,2,3, Liu X1,2,3.

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a College of Veterinary Medicine , Yangzhou University , Yangzhou , People's Republic of China.
b Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses , Yangzhou , People' Republic of China.
c Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease , Yangzhou , People' Republic of China.
d Faculty of Public and Environmental Health , University of Khartoum , Khartoum , Sudan.


Salmonella biofilm formation is important to environmental stress resistance and virulence. However, the roles of the csgA and bcsA genes, which affect curli protein and cellulose production, respectively, in Salmonella enterica serovar Pullorum, are unknown. Here we constructed deletions in the csgA and bcsA genes in S. enterica serovar Pullorum strain S6702 and evaluated several aspects of biofilm formation and virulence. ΔcsgA showed decreased production of curli fimbriae, while ΔbcsA had reduced cellulose production. Both mutants had a reduced ability to form biofilms. ΔcsgA was reduced in adhesion and invasion to HeLa cells and exhibited decreased intracellular proliferation in HD11 macrophages. ΔbcsA exhibited increased proliferation in HD11 cells and replicated better in chicken spleens, as compared to the wild-type strain. ΔcsgA virulence was attenuated in assays involving oral challenge of one-day-old chickens.


Salmonella enterica serovar Pullorum; bcsA; biofilm; csgA; mutant; pathogenicity

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