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Intrinsically Disord Proteins. 2017 Mar 7;5(1):e1264350. doi: 10.1080/21690707.2016.1264350. eCollection 2017.

On the potential of using peculiarities of the protein intrinsic disorder distribution in mitochondrial cytochrome b to identify the source of animal meats.

Author information

1
Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; Department of Cell Biology, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Gizza, Egypt.
2
Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; EgyBlood, Vacsera, Cairo, Egypt.
3
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia; Department of Molecular Medicine and USF Health Byrd Alzheimer Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.

Abstract

This study was conducted to identify the source of animal meat based on the peculiarities of protein intrinsic disorder distribution in mitochondrial cytochrome b (mtCyt-b). The analysis revealed that animal and avian species can be discriminated based on the proportions of the two groups of residues, Leu+Ile, and Ser+Pro+Ala, in the amino acid sequences of their mtCyt-b. Although levels of the overall intrinsic disorder in mtCyt-b is not very high, the peculiarities of disorder distribution within the sequences of mtCyt-b from different species varies in a rather specific way. In fact, positions and intensities of disorder/flexibility "signals" in the corresponding disorder profiles are relatively unique for avian and animal species. Therefore, it is possible to devise a set of simple rules based on the peculiarities of disorder profiles of their mtCyt-b proteins to discriminate among species. This intrinsic disorder-based analysis represents a new technique that could be used to provide a promising solution for identification of the source of meats.

KEYWORDS:

adulteration; animal species; authentication; avian species; food products; intrinsically disordered protein; intrinsically disordered region; meat; mitochondrial cytochrome b

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