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PLoS One. 2016 Aug 11;11(8):e0160853. doi: 10.1371/journal.pone.0160853. eCollection 2016.

A Public Database of Memory and Naive B-Cell Receptor Sequences.

Author information

1
Adaptive Biotechnologies, Seattle, United States of America.
2
Fred Hutchinson Cancer Research Center, Seattle, United States of America.
3
University of Washington, Seattle, United States of America.

Abstract

The vast diversity of B-cell receptors (BCR) and secreted antibodies enables the recognition of, and response to, a wide range of epitopes, but this diversity has also limited our understanding of humoral immunity. We present a public database of more than 37 million unique BCR sequences from three healthy adult donors that is many fold deeper than any existing resource, together with a set of online tools designed to facilitate the visualization and analysis of the annotated data. We estimate the clonal diversity of the naive and memory B-cell repertoires of healthy individuals, and provide a set of examples that illustrate the utility of the database, including several views of the basic properties of immunoglobulin heavy chain sequences, such as rearrangement length, subunit usage, and somatic hypermutation positions and dynamics.

PMID:
27513338
PMCID:
PMC4981401
DOI:
10.1371/journal.pone.0160853
[Indexed for MEDLINE]
Free PMC Article

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