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J Biomed Mater Res B Appl Biomater. 2017 Oct;105(7):1758-1766. doi: 10.1002/jbm.b.33652. Epub 2016 May 17.

Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.

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Department of Physics, Kasetsart University, Bangkok, Thailand.
Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand.
Department of Physiology, Mahidol University, Bangkok, Thailand.
Department of Materials Science, Kasetsart University, Bangkok, Thailand.


In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol). Porous scaffolds were obtained by freeze drying the combinations. The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated. The investigation indicated that the compressive strength was controlled by the types of CaP. The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite. Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively. In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface. Comparing the scaffolds, cells grown on the BCPBG based composite showed the higher cell density. To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells. The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased. Therefore, ChiCol-fabricated BCPBG scaffold shows promise for bone regeneration.


bioactive glass; biopolymers; bone scaffolds; calcium phosphate; mechanical properties

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