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Carbohydr Res. 2016 Jun 2;427:38-43. doi: 10.1016/j.carres.2016.03.026. Epub 2016 Apr 4.

The structure of the lipooligosaccharide from Xanthomonas oryzae pv. Oryzae: the causal agent of the bacterial leaf blight in rice.

Author information

1
Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy. Electronic address: flaviana.dilorenzo@unina.it.
2
CNR-Istituto per i Polimeri, Compositi e Biomateriali IPCB Unità di Catania, Via Gaifami 18, 95126 Catania, Italy.
3
Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy.
4
Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki 214-8571, Japan.
5
Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy. Electronic address: molinaro@unina.it.

Abstract

The structure of the lipooligosaccharide (LOS) from the rice pathogen Xanthomonas oryzae pv. oryzae has been elucidated. The characterization of the core oligosaccharide structure was obtained by the employment of two chemical degradation protocols and by analysis of the products via NMR spectroscopy. The structure of the lipid A portion was achieved by MALDI mass spectrometry analysis on purified lipid A. The LOS from Xanthomonas oryzae pv. oryzae revealed to possess the same core structure of Xanthomonas campestris pv. campestris and interesting novel features on its lipid A domain. The evaluation of the biological activity of both LOS and isolated lipid A was also executed.

KEYWORDS:

Bacterial leaf blight; Lipopolysaccharide (LPS); Plant immunity; Structural characterization; Xanthomonas genus

PMID:
27085742
DOI:
10.1016/j.carres.2016.03.026
[Indexed for MEDLINE]

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