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Artif Cells Nanomed Biotechnol. 2017 Mar;45(2):372-379. doi: 10.3109/21691401.2016.1160403. Epub 2016 Mar 29.

Green synthesis of silver nanoparticles using Rheum palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa.

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a Institute of Green Bioscience and Technology, Seoul National University , Pyeongchang , Republic of Korea.
b Centre for Environmental Research and Development, Loyola College , Chennai , India.
c Department of Medical Microbiology and Immunology , Institute of Biomedical Sciences, Mekelle University , Mekelle , Ethiopia.
d Department of Animal Nutrition and Physiology, National Institute of Animal Science , RDA, Jeonju , Republic of Korea.
e Graduate School of International Agricultural Technology, Seoul National University , Pyeongchang , Republic of Korea.


In the present study, silver nanoparticles (AgNPs) were synthesized by using aqueous root extracts of Rheum palmatum and characterized by various spectroscopic methods. The nanoparticles were found to be in hexagonal and spherical shapes. The average particle size was found to be 121 ± 2 nm with zeta potential values of -21.6 mv by dynamic light scattering (DLS) method. Gas chromatography-mass spectrometry (GC-MS) analysis of R. palmatum revealed 35 compounds. The synthesized AgNPs showed significant activity against Staphylococcus aureus and Pseudomonas aeruginosa with IC90 values of 15 μg/ml and IC50 values of 7.5 μg/ml, respectively. The protein leakage level was high and morphological changes occurred in bacteria treated with AgNPs.


Antibacterial; Rheum palmatum; SEM; XRD; green synthesis; silver nanoparticle

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