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J Chromatogr A. 2016 Apr 22;1443:21-5. doi: 10.1016/j.chroma.2016.03.032. Epub 2016 Mar 17.

Interrelationship between partition behavior of organic compounds and proteins in aqueous dextran-polyethylene glycol and polyethylene glycol-sodium sulfate two-phase systems.

Author information

1
Analiza, Inc., 3615 Superior Ave., Cleveland, OH 44114, USA.
2
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
3
Universidade de São Paulo, Faculdade de Ciencias Farmaceuticas, Laboratório de Biologia Molecular Aplicada à Biotecnologia Farmacêutica, São Paulo, Brazil.
4
LEPABE, Department of Chemical Engineering, Faculty of Engineering of the University of Porto, 4200-465 Porto, Portugal.
5
Centro de Investigacao em Materiais Ceramicos e Compositos, Department of Chemistry, Aveiro, Portugal, Portugal; Laboratory of Separation and Reaction Engineering-Laboratory of Catalysis and Materials (LSRE-LCM), Porto, Portugal.
6
Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
7
Analiza, Inc., 3615 Superior Ave., Cleveland, OH 44114, USA. Electronic address: bz@analiza.com.

Abstract

Partition behavior of adenosine and guanine mononucleotides was examined in aqueous dextran-polyethylene glycol (PEG) and PEG-sodium sulfate two-phase systems. The partition coefficients for each series of mononucleotides were analyzed as a functions of the number of phosphate groups and found to be dependent on the nature of nucleic base and on the type of ATPS utilized. It was concluded that an average contribution of a phosphate group into logarithm of partition coefficient of a mononucleotide cannot be used to estimate the difference between the electrostatic properties of the coexisting phases of ATPS. The data obtained in this study were considered together with those for other organic compounds and proteins reported previously, and the linear interrelationship between logarithms of partition coefficients in dextran-PEG, PEG-Na2SO4 and PEG-Na2SO4-0.215M NaCl (all in 0.01M Na- or K/Na-phosphate buffer, pH 7.4 or 6.8) was established. Similar relationship was found for the previously reported data for proteins in Dex-PEG, PEG-600-Na2SO4, and PEG-8000-Na2SO4 ATPS. It is suggested that the linear relationships of the kind established in ATPS may be observed for biological properties of compounds as well.

KEYWORDS:

Aqueous two phase systems; Aqueous two-phase partitioning; Dex-PEG system; Mononucleotides; PEG-sodium sulfate system; Phosphate group

PMID:
27016118
DOI:
10.1016/j.chroma.2016.03.032
[Indexed for MEDLINE]

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