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Toxicol In Vitro. 2016 Apr;32:166-80. doi: 10.1016/j.tiv.2015.12.010. Epub 2015 Dec 30.

Autophagic activity in BC3H1 cells exposed to yessotoxin.

Author information

1
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU) - Campus Ås, P.O. Box 5003, NO-1432 Ås, Norway. Electronic address: monica.suarez.korsnes@nmbu.no.
2
Imaging Centre, Norwegian University of Life Sciences (NMBU) - Campus Ås, P.O. Box 5003, NO-1432 Ås, Norway.
3
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU) - Campus Ås, P.O. Box 5003, NO-1432 Ås, Norway; Smerud Medical Research, Oslo, Norway.
4
Norwegian Institute of Bioeconomy Research (NIBIO), Ås, Norway; Norwegian Defense Research Establishment (FFI), Kjeller, Norway.

Abstract

The marine toxin yessotoxin (YTX) can induce programmed cell death through both caspase-dependent and -independent pathways in various cellular systems. It appears to stimulate different forms of cellular stress causing instability among cell death mechanisms and making them overlap and cross-talk. Autophagy is one of the key pathways that can be stimulated by multiple forms of cellular stress which may determine cell survival or death. The present work evaluates a plausible link between ribotoxic stress and autophagic activity in BC3H1 cells treated with YTX. Such treatment produces massive cytoplasmic compartments as well as double-membrane vesicles termed autophagosomes which are typically observed in cells undergoing autophagy. The observed autophagosomes contain a large amount of ribosomes associated with the endoplasmic reticulum (ER). Western blotting analysis of Atg proteins and detection of the autophagic markers LC3-II and SQSTM1/p62 by flow cytometry and immunofluorescence verified autophagic activity during YTX-treatment. The present work supports the idea that autophagic activity upon YTX exposure may represent a response to ribotoxic stress.

KEYWORDS:

Atg proteins; Autophagosomes; Autophagy; BC3H1 cells; LC3-II; Lamellar bodies; Yessotoxin

PMID:
26743762
DOI:
10.1016/j.tiv.2015.12.010
[Indexed for MEDLINE]
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