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Environ Sci Pollut Res Int. 2017 Apr;24(10):9048-9057. doi: 10.1007/s11356-015-5891-7. Epub 2015 Dec 29.

Seasonal variation and removal efficiency of antibiotic resistance genes during wastewater treatment of swine farms.

Sui Q1,2, Zhang J1,2, Tong J1,2, Chen M3,4, Wei Y5,6,7.

Author information

1
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
2
Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
3
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. mxchen@rcees.ac.cn.
4
Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. mxchen@rcees.ac.cn.
5
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. yswei@rcees.ac.cn.
6
Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. yswei@rcees.ac.cn.
7
Institute of Energy, Jiangxi Academy of Sciences, Nanchang, 330096, China. yswei@rcees.ac.cn.

Abstract

The seasonal variation and removal efficiency of antibiotic resistance genes (ARGs), including tetracycline resistance genes (tetG, tetM, and tetX) and macrolide (ermB, ermF, ereA, and mefA), were investigated in two typical swine wastewater treatment systems in both winter and summer. ARGs, class 1 integron gene, and 16S rRNA gene were quantified using real-time polymerase chain reaction assays. There was a 0.31-3.52 log variation in ARGs in raw swine wastewater, and the abundance of ARGs in winter was higher than in summer. tetM, tetX, ermB, ermF, and mefA were highly abundant. The abundance of ARGs was effectively reduced by most individual treatment process and the removal efficiencies of ARGs were higher in winter than in summer. However, when examining relative abundance, the fate of ARGs was quite variable. Anaerobic digestion reduced the relative abundance of tetX, ermB, ermF, and mefA, while lagoon treatment decreased tetM, ermB, ermF, and mefA. Sequencing batch reactor (SBR) decreased tetM, ermB, and ermF, but biofilters and wetlands did not display consistent removal efficiency on ARGs in two sampling seasons. As far as the entire treatment system is concerned, ermB and mefA were effectively reduced in both winter and summer in both total and relative abundance. The relative abundances of tetG and ereA were significantly correlated with intI1 (pā€‰<ā€‰0.01), and both tetG and ereA increased after wastewater treatment. This may pose a great threat to public health.

KEYWORDS:

Antibiotic resistance genes; Antibiotic resistant bacteria; Biological wastewater treatment; Class 1 integron; Real-time polymerase chain reaction; Swine wastewater

PMID:
26715413
DOI:
10.1007/s11356-015-5891-7
[Indexed for MEDLINE]

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