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BMC Infect Dis. 2015 Nov 25;15:544. doi: 10.1186/s12879-015-1281-5.

Detection of HPV DNA in paraffin-embedded cervical samples: a comparison of four genotyping methods.

Author information

1
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. f.castro@Dkfz-Heidelberg.de.
2
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. koshiolj@mail.nih.gov.
3
DDL, Diagnostic Laboratory, Rijswijk, The Netherlands. wim.quint@ddl.nl.
4
Department of Pathology, School of Medicine, University of New Mexico, Albuquerque, NM, USA. CWheeler@salud.unm.edu.
5
Viral Oncology Program, The Ohio State University Comprehensive Cancer Center, Columbus, OH, USA. Maura.Gillison@osumc.edu.
6
BD Diagnostics, Sparks, MD, USA. laurence_vaughan@bd.com.
7
DDL, Diagnostic Laboratory, Rijswijk, The Netherlands. Bernhard.Kleter@ddl.nl.
8
DDL, Diagnostic Laboratory, Rijswijk, The Netherlands. L.J.van.Doorn@ddl.nl.
9
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. chaturva@mail.nih.gov.
10
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. hildesha@exchange.nih.gov.
11
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. schiffmm@exchange.nih.gov.
12
Division of Cancer Etiology, Department of Population Sciences, Beckman Research Institute and the City of Hope, Duarte, CA, USA. sowang@coh.org.
13
Departments of Pathology and Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. rosemary-zuna@ouhsc.edu.
14
Departments of Pathology and Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. joan-walker@ouhsc.edu.
15
Departments of Pathology and Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. Terence-Dunn@ouhsc.edu.
16
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. wentzenn@mail.nih.gov.
17
Division of Cancer Epidemiology & Genetics, NCI Shady Grove, 9609 Medical Center Drive 7E114, Rockville, MD, 20850, USA. wentzenn@mail.nih.gov.

Abstract

BACKGROUND:

Identification of human papillomavirus (HPV) DNA in cervical tissue is important for understanding cervical carcinogenesis and for evaluating cervical cancer prevention approaches. However, HPV genotyping using formalin-fixed, paraffin-embedded (FFPE) tissues is technically challenging. We evaluated the performance of four commonly used genotyping methods on FFPE cervical specimens conducted in different laboratories and compared to genotyping results from cytological samples.

METHODS:

We included 60 pairs of exfoliated-cell and FFPE specimens from women with histologically confirmed cervical intraepithelial lesions grade 2 or 3. Cytology specimens were genotyped using the Linear Array assay. Four expert laboratories processed tissue specimens using different preparation methods and then genotyped the resultant sample preparations using four different HPV genotyping methods: SPF10-PCR DEIA LiPA25 (version 1), Inno-LiPA, Linear Array and the Onclarity assay. Percentage agreement, kappa statistics and McNemar's chi-square were calculated for each comparison of different methods and specimen types.

RESULTS:

Overall agreement with respect to carcinogenic HPV status for FFPE samples between different methods was: 81.7, 86.7 and 91.7% for Onclarity versus Inno-LiPA, Linear Array and SPF-LiPA25, respectively; 81.7 and 85.0% for Linear Array versus Inno-LiPA and SPF-LiPA25, respectively; and 86.7% for SPF-LiPA25 versus Inno-LiPA. Type-specific agreement was >88.3% for all pair-wise comparisons. Comparisons with cytology specimens resulted in overall agreements from 80 to 95% depending on the method and type-specific agreement was >90% for most comparisons.

CONCLUSIONS:

Our data demonstrate that the four genotyping methods run by expert laboratories reliably detect HPV DNA in FFPE specimens with some variation in genotype-specific detection.

PMID:
26607224
PMCID:
PMC4660657
DOI:
10.1186/s12879-015-1281-5
[Indexed for MEDLINE]
Free PMC Article

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