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Lab Anim. 2015 Jul;49(2 Suppl):1-90. doi: 10.1177/0023677215580006.

Guidelines for the Care and Welfare of Cephalopods in Research -A consensus based on an initiative by CephRes, FELASA and the Boyd Group.

Author information

1
Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy Association for Cephalopod Research 'CephRes', Italy cephres@cephalopodresearch.org.
2
Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy Animal Model Facility - BIOGEM S.C.A.R.L., Ariano Irpino (AV), Italy.
3
Biology Department, Brooklyn College - CUNY Graduate Center, Brooklyn, NY, USA.
4
Association for Cephalopod Research 'CephRes', Italy.
5
Department of Veterinary Medicine and Animal Productions - University of Naples Federico II, Napoli, Italy AISAL - Associazione Italiana per le Scienze degli Animali da Laboratorio, Milano, Italy.
6
Groupe mémoire et Plasticité comportementale, University of Caen Basse-Normandy, Caen, France.
7
Instituto de Investigaciones Marinas (IIM-CSIC), Vigo, Spain.
8
BioMimetic and Cognitive Robotics, Department of Psychology, Brooklyn College - CUNY, Brooklyn, NY, USA.
9
Max Planck Institute for Brain Research, Frankfurt, Germany.
10
Integrative Ecophysiology, Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany.
11
Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
12
School of Life Sciences, University of Sussex, Sussex, UK.
13
Department of Life Sciences, Eilat Campus, Ben-Gurion University of the Negev, Beer, Sheva, Israel.
14
FELASA, Federation for Laboratory Animal Science Associations.
15
The Boyd Group, Hereford, UK.
16
Division of Biomedical Sciences, St George's University of London, London, UK Association for Cephalopod Research 'CephRes', Italy.

Abstract

This paper is the result of an international initiative and is a first attempt to develop guidelines for the care and welfare of cephalopods (i.e. nautilus, cuttlefish, squid and octopus) following the inclusion of this Class of ∼700 known living invertebrate species in Directive 2010/63/EU. It aims to provide information for investigators, animal care committees, facility managers and animal care staff which will assist in improving both the care given to cephalopods, and the manner in which experimental procedures are carried out. Topics covered include: implications of the Directive for cephalopod research; project application requirements and the authorisation process; the application of the 3Rs principles; the need for harm-benefit assessment and severity classification. Guidelines and species-specific requirements are provided on: i. supply, capture and transport; ii. environmental characteristics and design of facilities (e.g. water quality control, lighting requirements, vibration/noise sensitivity); iii. accommodation and care (including tank design), animal handling, feeding and environmental enrichment; iv. assessment of health and welfare (e.g. monitoring biomarkers, physical and behavioural signs); v. approaches to severity assessment; vi. disease (causes, prevention and treatment); vii. scientific procedures, general anaesthesia and analgesia, methods of humane killing and confirmation of death. Sections covering risk assessment for operators and education and training requirements for carers, researchers and veterinarians are also included. Detailed aspects of care and welfare requirements for the main laboratory species currently used are summarised in Appendices. Knowledge gaps are highlighted to prompt research to enhance the evidence base for future revision of these guidelines.

KEYWORDS:

3Rs; Cephalopods; Directive 2010/63/EU; animal welfare; invertebrates

PMID:
26354955
DOI:
10.1177/0023677215580006
[Indexed for MEDLINE]

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