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Urol J. 2015 Jul 1;12(3):2160-4.

A Significant Upregulation of miR-886-5p in High Grade and Invasive Bladder Tumors.

Author information

1
Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2
Department of Pathology, Labbafi-Nejad Medical Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3
Department of Anatomy, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
4
Urology and Nephrology Research Center, Labbafi-Nejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. samziaee@gmail.com.
5
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. sjmowla@modares.ac.ir.

Abstract

PURPOSE:

To investigate the expression alteration of miR-886-5p in bladder tumors and evaluating its expression level as a potential biomarker in this type of cancer.

MATERIALS AND METHODS:

Formalin-fixed paraffin-embedded (FFPE) samples of bladder tumors belonging to 70 patients whom had been referred to the Shahid Labbafi-Nejad medical center were obtained from the archival collection of pathology department. After RNA extraction and cDNA synthesis, expression levels of miR-886-5p were quantified by a real-time reverse transcription polymerase chain reaction (RT-PCR) approach.

RESULTS:

Our data revealed a significant upregulation (~3 times) of miR-886-5p in high grade bladder tumors, compared to the low grade ones (P < .05). Moreover, its expression level could significantly discriminate noninvasive (Ta, T1) from invasive (T-2T4) tumor stages.

CONCLUSION:

Our data suggests a potential role for miR-886-5p in progression of bladder cancer.

PMID:
26135932
[Indexed for MEDLINE]
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