Format

Send to

Choose Destination
Bioinformatics. 2015 Oct 1;31(19):3210-2. doi: 10.1093/bioinformatics/btv351. Epub 2015 Jun 9.

BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.

Author information

1
Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.

Abstract

MOTIVATION:

Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.

RESULTS:

We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.

AVAILABILITY AND IMPLEMENTATION:

Software implemented in Python and datasets available for download from http://busco.ezlab.org.

CONTACT:

evgeny.zdobnov@unige.ch

SUPPLEMENTARY INFORMATION:

Supplementary data are available at Bioinformatics online.

PMID:
26059717
DOI:
10.1093/bioinformatics/btv351
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center