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Cell Metab. 2015 Jun 2;21(6):891-7. doi: 10.1016/j.cmet.2015.04.011. Epub 2015 May 7.

Metabolism links bacterial biofilms and colon carcinogenesis.

Author information

1
Scripps Center for Metabolomics and Mass Spectrometry, The Scripps Research Institute, La Jolla, CA 92037, USA.
2
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Baltimore, MD 21218, USA.
3
Center of Surgical Gastroenterology, Karolinska University Hospital, 171 77 Stockholm, Sweden.
4
Departments of Chemical Physiology and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
5
Departments of Chemistry, Genetics, and Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
6
Department of Surgery, Johns Hopkins University School of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21218, USA.
7
Department of Surgery, Scripps Clinic Medical Group, La Jolla, CA 92037, USA.
8
Departments of Oncology and Medicine and the Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21218, USA.
9
Resphera Biosciences, Baltimore, MD 21231, USA.
10
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Baltimore, MD 21218, USA; Departments of Oncology and Medicine and the Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21218, USA. Electronic address: csears@jhmi.edu.
11
Scripps Center for Metabolomics and Mass Spectrometry, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: siuzdak@scripps.edu.

Abstract

Bacterial biofilms in the colon alter the host tissue microenvironment. A role for biofilms in colon cancer metabolism has been suggested but to date has not been evaluated. Using metabolomics, we investigated the metabolic influence that microbial biofilms have on colon tissues and the related occurrence of cancer. Patient-matched colon cancers and histologically normal tissues, with or without biofilms, were examined. We show the upregulation of polyamine metabolites in tissues from cancer hosts with significant enhancement of N(1), N(12)-diacetylspermine in both biofilm-positive cancer and normal tissues. Antibiotic treatment, which cleared biofilms, decreased N(1), N(12)-diacetylspermine levels to those seen in biofilm-negative tissues, indicating that host cancer and bacterial biofilm structures contribute to the polyamine metabolite pool. These results show that colonic mucosal biofilms alter the cancer metabolome to produce a regulator of cellular proliferation and colon cancer growth potentially affecting cancer development and progression.

PMID:
25959674
PMCID:
PMC4456201
DOI:
10.1016/j.cmet.2015.04.011
[Indexed for MEDLINE]
Free PMC Article

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