Format

Send to

Choose Destination
J Radiat Res. 2015 May;56(3):437-45. doi: 10.1093/jrr/rru121. Epub 2015 Feb 4.

Generation of peanut mutants by fast neutron irradiation combined with in vitro culture.

Author information

1
College of Life Sciences, Qingdao Agricultural University/Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao 266109, China.
2
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China.
3
Shandong Agricultural Broadcasting and Television School, Penglai 265600, China.
4
Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, China.
5
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China liuluxiang@caas.cn.

Abstract

Induced mutations have played an important role in the development of new plant varieties. In this study, we investigated the effects of fast neutron irradiation on somatic embryogenesis combined with plant regeneration in embryonic leaflet culture to develop new peanut (Arachis hypogaea L.) germplasm for breeding. The dry seeds of the elite cultivar Luhua 11 were irradiated with fast neutrons at dosages of 9.7, 14.0 and 18.0 Gy. The embryonic leaflets were separated and incubated in a medium with 10.0-mg/l 2,4-D to induce somatic embryogenesis. Next, they were incubated in a medium with 4.0-mg/l BAP for plant regeneration. As the irradiation dosage increased, the frequency of both somatic embryo formation and plantlet regeneration decreased. The regenerated plantlets were grafted onto rootstocks and were transplanted into the field. Later, the mature seeds of the regenerated plants were harvested. The M2 generation plants from most of the regenerated cultivars exhibited variations and segregation in vigor, plant height, branch and pod number, pod size, and pod shape. To determine whether the phenotypes were associated with genomic modification, we compared the DNA polymorphisms between the wild-type plants and 19 M3-generation individuals from different regenerated plants. We used 20 pairs of simple sequence repeat (SSR) primers and detected polymorphisms between most of the mutants and the wild-type plants (Luhua 11). Our results indicate that using a combination of fast neutron irradiation and tissue culture is an effective approach for creating new peanut germplasm.

KEYWORDS:

fast neutron irradiation; in vitro culture; mutagenesis; mutant; peanut (Arachis hypogaea L.)

PMID:
25653418
PMCID:
PMC4426915
DOI:
10.1093/jrr/rru121
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center