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Dev Comp Immunol. 2015 Jan;48(1):22-32. doi: 10.1016/j.dci.2014.08.011. Epub 2014 Sep 6.

Toll-like receptor 2 ligand, lipoteichoic acid is inhibitory against infectious laryngotracheitis virus infection in vitro and in vivo.

Author information

1
Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C64, 3330 Hospital Drive NW, Calgary, Canada AB T2N 2Z6.
2
Department of Pathobiology, University of Guelph, Guelph, Canada.
3
Veterinary Science Research Station, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.
4
Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C64, 3330 Hospital Drive NW, Calgary, Canada AB T2N 2Z6. Electronic address: faizal.abdulcareem@ucalgary.ca.

Abstract

Lipoteichoic acid (LTA) is one of the pathogen associated molecular patterns (PAMPs) that activates toll-like receptor (TLR)2-cluster of differentiation (CD)14 signalling pathway. This recognition elicits antiviral responses that have been recorded against viruses of mammals although such responses have not been characterized adequately against avian viruses. In this investigation, we characterized the LTA induced antiviral responses against infectious laryntotracheitis virus (ILTV) infection in vitro and in vivo. We found that LTA is capable of up regulating mRNA expression of innate proteins in macrophages such as MyD88, iNOS and IL-1β and reduces the ILTV plaques in vitro. Similarly, we found that LTA treatment of embryonic day 18 (ED18) eggs can lead to the antiviral response against pre-hatch ILTV infection in vivo and is associated with expansion of macrophage populations and expression of IL-1β and MyD88 in the lung. The data highlight that LTA can be a potential innate immune stimulant that can be used against ILTV infection in chickens.

KEYWORDS:

In ovo; Infectious laryngotracheitis virus; Lipoteichoic acid; Macrophage; Nitric oxide; Toll-like receptor 2

PMID:
25195716
DOI:
10.1016/j.dci.2014.08.011
[Indexed for MEDLINE]

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