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Antiviral Res. 2014 Sep;109:22-9. doi: 10.1016/j.antiviral.2014.06.008. Epub 2014 Jun 26.

Swine interferon-induced transmembrane protein, sIFITM3, inhibits foot-and-mouth disease virus infection in vitro and in vivo.

Author information

1
State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Division of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
2
Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou 730046, China.
3
State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Division of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: lixiangmin@mail.hzau.edu.cn.

Abstract

The interferon-induced transmembrane protein 3 (IFITM3) is a widely expressed potent antiviral effector of the host innate immune system. It restricts a diverse group of pathogenic, enveloped viruses, by interfering with endosomal fusion. In this report, the swine IFITM3 (sIFITM3) gene was cloned. It shares the functionally conserved CD225 domain and multiple critical amino acid residues (Y19, F74, F77, R86 and Y98) with its human ortholog, which are essential for antiviral activity. Ectopic expression of sIFITM3 significantly inhibited non-enveloped foot-and-mouth disease virus (FMDV) infection in BHK-21 cells. Furthermore, sIFITM3 blocked FMDV infection at early steps in the virus life cycle by disrupting viral attachment to the host cell surface. Importantly, inoculation of 2-day-old suckling mice with a plasmid expressing sIFITM3 conferred protection against lethal challenge with FMDV. These results suggest that sIFITM3 is a promising antiviral agent and that can safeguard the host from infection with FMDV.

KEYWORDS:

Antiviral activity; Foot-and-mouth disease virus; Swine IFITM3; Viral attachment

PMID:
24973762
DOI:
10.1016/j.antiviral.2014.06.008
[Indexed for MEDLINE]

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