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J Immunol Methods. 1988 Nov 10;114(1-2):49-52.

An immunisation protocol which enhances the frequency of antigen-specific monoclonal antibody production.

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Department of Biochemistry, University of Surrey, Guildford, U.K.


An immunisation protocol has been developed for small molecular weight antigens which results in a high percentage of specific hybridomas being produced after cell fusion. An enzyme-linked immunosorbent assay (ELISA) was developed for screening the desired antibodies in the culture supernatants. A conventional immunisation regimen was followed by doses of antigen in sterile water on each of the last 4 days before fusion. A range of antigen doses was used and the specific efficiency of fusion was increased by selection of the optimum amount. The antigen used as a model antigen in these experiments was biosynthetic human insulin.

[Indexed for MEDLINE]

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