Comparison of IncFII-type plasmids pMC-NDM and pGUE-NDM carrying bla_NDM-1 genes. Boxes indicate ORFs identified by sequence analysis. All regions are drawn to scale, except for the tra-trb locus. Gene designations refer to ORFs with putative functions that were assigned on the basis of homology searches performed by Bonnin et al. () or in this study. Parts of both plasmids showing similar structures and high sequence homology are indicated by frames shaded light gray. Dark gray and black boxes indicate ORFs found only in pGUE-NDM and pMC-NDM, respectively. The bla_NDM-1 genes are represented by hatched boxes. The IncFII backbone, mosaic regions, and the pMC-NDM fragments that are highly homologous to plasmids pSal8934a and pXD2 are indicated by horizontal arrows.