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PeerJ. 2013 Jul 9;1:e101. doi: 10.7717/peerj.101. Print 2013.

Looking at microbial metabolism by high-resolution (2)H-NMR spectroscopy.

Author information

1
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences , Pushchino , Russia.

Abstract

We analyzed the applicability of high-resolution (2)H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

KEYWORDS:

2H-HMR spectroscopy; Heavy water; High resolution NMR; Microbe adaptation; Microbial metabolism

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