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Virus Genes. 2013 Oct;47(2):219-27. doi: 10.1007/s11262-013-0939-6. Epub 2013 Jun 28.

Activation of elements in HERV-W family by caffeine and aspirin.

Author information

1
Department of Medical Microbiology, School of Medicine, Wuhan University, 185 Donghu Road, Wuhan, 430071, People's Republic of China.

Abstract

Caffeine and aspirin have been suggested to be involved in neurologic diseases, such as schizophrenia, and previous data have revealed that abnormal expression of HERV-W elements may be an important factor in the etiopathogenesis of those diseases. In this article, we reported that caffeine and aspirin contributed to the expression of HERV-W env and gag in Human SH-SY5Y neuroblastoma cells. Semi-quantitative RT-PCR and quantitative Real-time PCR were used to detect the mRNA of HERV-W env and gag in cells exposed to caffeine or aspirin. Western blotting was used to detect the protein of HERV-W env. Luciferase activity assay was employed to detect the activity of HERV-W env promoter. It was found that both caffeine and aspirin could increase the expression of HERV-W env and gag in human SH-SY5Y neuroblastoma cells. Caffeine could activate the HERV-W env promoter, while aspirin could not. With previous studies we can conjecture thatĀ HERVs might play a bridging role between environmental factors, such as drugs and neurologic diseases.

PMID:
23813246
DOI:
10.1007/s11262-013-0939-6
[Indexed for MEDLINE]

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