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FEBS Lett. 2013 Jun 5;587(11):1587-91. doi: 10.1016/j.febslet.2013.04.006. Epub 2013 Apr 18.

Stochastic machines as a colocalization mechanism for scaffold protein function.

Author information

1
Department of Molecular Medicine, University of South Florida, Tampa, FL 33612, USA.

Abstract

The axis inhibition (Axin) scaffold protein colocalizes β-catenin, casein kinase Iα, and glycogen synthetase kinase 3β by their binding to Axin's long intrinsically disordered region, thereby yielding structured domains with flexible linkers. This complex leads to the phosphorylation of β-catenin, marking it for destruction. Fusing proteins with flexible linkers vastly accelerates chemical interactions between them by their colocalization. Here we propose that the complex works by random movements of a "stochastic machine," not by coordinated conformational changes. This non-covalent, modular assembly process allows the various molecular machine components to be used in multiple processes.

PMID:
23603389
PMCID:
PMC4141485
DOI:
10.1016/j.febslet.2013.04.006
[Indexed for MEDLINE]
Free PMC Article

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