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Biochim Biophys Acta. 2013 Feb;1834(2):583-92. doi: 10.1016/j.bbapap.2012.11.004. Epub 2012 Nov 19.

High throughput characterization of structural differences between closely related proteins in solution.

Author information

1
Analiza, Inc., 3516 Superior Ave., Suite 4407B, Cleveland, OH 44114, USA.

Abstract

Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired.

PMID:
23174655
DOI:
10.1016/j.bbapap.2012.11.004
[Indexed for MEDLINE]

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